FLOT1
Flotillin-1
Also known as: FLOT1_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O75955
- Gene
- FLOT1
- Ensembl
- ENSG00000137312
- Chromosome
- 6
- Canonical length
- 427 aa
- Protein class
- Plasma proteins, Predicted intracellular proteins, Transporters
- Subcellular location
- Golgi apparatus,Vesicles,Plasma membrane
OverviewNCBI Gene
This gene encodes an protein that localizes to the caveolae, which are small domains on the inner cell membranes. This protein plays a role in vesicle trafficking and cell morphology. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
Canonical amino-acid sequenceUniProt
427 residues, UniProt reviewed canonical sequence.
>O75955|FLOT1
1 MFFTCGPNEA MVVSGFCRSP PVMVAGGRVF VLPCIQQIQR ISLNTLTLNV KSEKVYTRHG
61 VPISVTGIAQ VKIQGQNKEM LAAACQMFLG KTEAEIAHIA LETLEGHQRA IMAHMTVEEI
121 YKDRQKFSEQ VFKVASSDLV NMGISVVSYT LKDIHDDQDY LHSLGKARTA QVQKDARIGE
181 AEAKRDAGIR EAKAKQEKVS AQYLSEIEMA KAQRDYELKK AAYDIEVNTR RAQADLAYQL
241 QVAKTKQQIE EQRVQVQVVE RAQQVAVQEQ EIARREKELE ARVRKPAEAE RYKLERLAEA
301 EKSQLIMQAE AEAASVRMRG EAEAFAIGAR ARAEAEQMAK KAEAFQLYQE AAQLDMLLEK
361 LPQVAEEISG PLTSANKITL VSSGSGTMGA AKVTGEVLDI LTRLPESVER LTGVSISQVN
421 HKPLRTALocalizationUniProt · AlphaFold · HPA
Whether an antibody against FLOT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.43
- Highest tissue expression
- 229 nTPM
Expression across tissuesHPA
Tissue
- adrenal gland: 229 nTPM
- heart muscle: 222 nTPM
- cerebral cortex: 185 nTPM
- spinal cord: 184 nTPM
- skeletal muscle: 177 nTPM
- midbrain: 162 nTPM
Single-cell type
- oocytes: 226 nCPM
- late spermatids: 140 nCPM
- neutrophils: 132 nCPM
- microglia: 82 nCPM
- late primary spermatocytes: 78 nCPM
- choroid plexus epithelial cells: 74 nCPM
Immune cell
- neutrophil: 315 nTPM
- basophil: 180 nTPM
- eosinophil: 132 nTPM
- NK-cell: 80 nTPM
- classical monocyte: 77 nTPM
- plasmacytoid DC: 59 nTPM
Brain region
- cerebral cortex: 30 nTPM
- hypothalamus: 28 nTPM
- choroid plexus: 26 nTPM
- pons: 25 nTPM
- basal ganglia: 21 nTPM
- hippocampal formation: 17 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.93
- gnomAD pLI
- 0
- gnomAD missense Z
- 2.34
- DepMap mean gene effect
- -0.19
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- adherens junction organization
- axonogenesis
- caveola assembly
- cell-cell adhesion mediated by cadherin
- extracellular matrix disassembly
- intracellular signal transduction
- plasma membrane raft assembly
- plasma membrane raft organization
- positive regulation of canonical NF-kappaB signal transduction
- positive regulation of cell junction assembly
- positive regulation of cell-cell adhesion mediated by cadherin
- positive regulation of endocytosis
- positive regulation of synaptic transmission, dopaminergic
- protein localization to plasma membrane
- regulation of neurotransmitter uptake
- regulation of receptor internalization
- regulation of Rho protein signal transduction
Molecular functions
Cellular components
- adherens junction
- basolateral plasma membrane
- caveola
- cell-cell contact zone
- cell-cell junction
- centriolar satellite
- cytoplasmic vesicle
- dopaminergic synapse
- early endosome
- endosome
- external side of plasma membrane
- extracellular exosome
- flotillin complex
- focal adhesion
- GABA-ergic synapse
- glutamatergic synapse
- lamellipodium
- lysosomal membrane
- melanosome
- membrane
- membrane raft
- microtubule organizing center
- plasma membrane
- presynaptic active zone
- sarcolemma
- uropod
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of FLOT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads FLOT1 as an antibody target. Whether an autoantibody or antibody against FLOT1 could matter depends on whether native FLOT1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
FLOT1 is annotated at the cell surface, where native FLOT1 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label FLOT1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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