FASTK

Protein identityUniProt · HPA

UniProt accession

Q14296

Gene

FASTK

Ensembl

ENSG00000164896

Chromosome

7

Canonical length

549 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Nuclear speckles,Mitochondria

OverviewNCBI Gene

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase was shown to be activated rapidly during Fas-mediated apoptosis in Jurkat cells. In response to Fas receptor ligation, it phosphorylates TIA1, an apoptosis-promoting nuclear RNA-binding protein. The encoded protein is a strong inducer of lymphocyte apoptosis. Two transcript variants encoding different isoforms have been found for this gene. Other variants exist, but their full-length natures have not yet been determined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

549 residues, UniProt reviewed canonical sequence.

>Q14296|FASTK
     1  MRRPRGEPGP RAPRPTEGAT CAGPGESWSP SPNSMLRVLL SAQTSPARLS GLLLIPPVQP
    61  CCLGPSKWGD RPVGGGPSAG PVQGLQRLLE QAKSPGELLR WLGQNPSKVR AHHYSVALRR
   121  LGQLLGSRPR PPPVEQVTLQ DLSQLIIRNC PSFDIHTIHV CLHLAVLLGF PSDGPLVCAL
   181  EQERRLRLPP KPPPPLQPLL RGGQGLEAAL SCPRFLRYPR QHLISSLAEA RPEELTPHVM
   241  VLLAQHLARH RLREPQLLEA IAHFLVVQET QLSSKVVQKL VLPFGRLNYL PLEQQFMPCL
   301  ERILAREAGV APLATVNILM SLCQLRCLPF RALHFVFSPG FINYISGTPH ALIVRRYLSL
   361  LDTAVELELP GYRGPRLPRR QQVPIFPQPL ITDRARCKYS HKDIVAEGLR QLLGEEKYRQ
   421  DLTVPPGYCT DFLLCASSSG AVLPVRTQDP FLPYPPRSCP QGQAASSATT RDPAQRVVLV
   481  LRERWHFCRD GRVLLGSRAL RERHLGLMGY QLLPLPFEEL ESQRGLPQLK SYLRQKLQAL
   541  GLRWGPEGG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against FASTK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.35

Highest tissue expression

115 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.58

gnomAD pLI

0

gnomAD missense Z

2.11

DepMap mean gene effect

0

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic signaling pathway
• mitochondrial RNA processing
• protein phosphorylation
• regulation of mitochondrial mRNA stability
• regulation of RNA splicing

Molecular functions

• ATP binding
• protein serine kinase activity
• protein serine/threonine kinase activity
• RNA binding
• Fas-activated serine/threonine kinase activity

Cellular components

• mitochondrial matrix
• mitochondrion
• ribonucleoprotein granule

Protein domainsUniProt · Pfam · InterPro

• FAST kinase leucine-rich
• FAST kinase-like protein, subdomain 2
• RAP domain
• FAST kinase domain-containing
• FAST kinase-like protein, subdomain 1
• FAST kinase-like protein, subdomain 2
• RAP domain

KeywordsUniProt

• Alternative initiation
• Apoptosis
• ATP-binding
• Kinase
• Mitochondrion
• Nucleotide-binding
• Phosphoprotein
• RNA-binding
• Serine/threonine-protein kinase
• Transferase

InteractionsUniProt · HPA

Protein binding partners of FASTK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• TIA1
• TIAR
• TIAL1