E2F2

Protein identityUniProt · HPA

UniProt accession

Q14209

Gene

E2F2

Ensembl

ENSG00000007968

Chromosome

1

Canonical length

437 aa

Protein class

Plasma proteins, Predicted intracellular proteins, Transcription factors

OverviewNCBI Gene

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

437 residues, UniProt reviewed canonical sequence.

>Q14209|E2F2
     1  MLQGPRALAS AAGQTPKVVP AMSPTELWPS GLSSPQLCPA TATYYTPLYP QTAPPAAAPG
    61  TCLDATPHGP EGQVVRCLPA GRLPAKRKLD LEGIGRPVVP EFPTPKGKCI RVDGLPSPKT
   121  PKSPGEKTRY DTSLGLLTKK FIYLLSESED GVLDLNWAAE VLDVQKRRIY DITNVLEGIQ
   181  LIRKKAKNNI QWVGRGMFED PTRPGKQQQL GQELKELMNT EQALDQLIQS CSLSFKHLTE
   241  DKANKRLAYV TYQDIRAVGN FKEQTVIAVK APPQTRLEVP DRTEDNLQIY LKSTQGPIEV
   301  YLCPEEVQEP DSPSEEPLPS TSTLCPSPDS AQPSSSTDPS IMEPTASSVP APAPTPQQAP
   361  PPPSLVPLEA TDSLLELPHP LLQQTEDQFL SPTLACSSPL ISFSPSLDQD DYLWGLEAGE
   421  GISDLFDSYD LGDLLIN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against E2F2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.61

Highest tissue expression

24 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.56

gnomAD pLI

0.26

gnomAD missense Z

1

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intrinsic apoptotic signaling pathway by p53 class mediator
• lens fiber cell apoptotic process
• negative regulation of sprouting angiogenesis
• positive regulation of transcription by RNA polymerase II
• regulation of cell cycle
• regulation of DNA-templated transcription
• regulation of transcription by RNA polymerase II
• transcription initiation at RNA polymerase II promoter

Molecular functions

• cis-regulatory region sequence-specific DNA binding
• DNA binding
• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• protein dimerization activity
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• nucleoplasm
• Rb-E2F complex
• RNA polymerase II transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• E2F/DP family, winged-helix DNA-binding domain
• E2F Family
• E2F transcription factor, CC-MB domain
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• E2F-DP heterodimerization region
• E2F/DP family winged-helix DNA-binding domain
• E2F transcription factor CC-MB domain

KeywordsUniProt

• Activator
• Cell cycle
• DNA-binding
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of E2F2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• EAPP
• RB1
• TFDP1