DHX29

Protein identityUniProt · HPA

UniProt accession

Q7Z478

Gene

DHX29

Ensembl

ENSG00000067248

Chromosome

5

Canonical length

1369 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells. [provided by RefSeq, Sep 2016]

Canonical amino-acid sequenceUniProt

1369 residues, UniProt reviewed canonical sequence.

>Q7Z478|DHX29
     1  MGGKNKKHKA PAAAVVRAAV SASRAKSAEA GIAGEAQSKK PVSRPATAAA AAAGSREPRV
    61  KQGPKIYSFN STNDSSGPAN LDKSILKVVI NNKLEQRIIG VINEHKKQNN DKGMISGRLT
   121  AKKLQDLYMA LQAFSFKTKD IEDAMTNTLL YGGDLHSALD WLCLNLSDDA LPEGFSQEFE
   181  EQQPKSRPKF QSPQIQATIS PPLQPKTKTY EEDPKSKPKK EEKNMEVNMK EWILRYAEQQ
   241  NEEEKNENSK SLEEEEKFDP NERYLHLAAK LLDAKEQAAT FKLEKNKQGQ KEAQEKIRKF
   301  QREMETLEDH PVFNPAMKIS HQQNERKKPP VATEGESALN FNLFEKSAAA TEEEKDKKKE
   361  PHDVRNFDYT ARSWTGKSPK QFLIDWVRKN LPKSPNPSFE KVPVGRYWKC RVRVIKSEDD
   421  VLVVCPTILT EDGMQAQHLG ATLALYRLVK GQSVHQLLPP TYRDVWLEWS DAEKKREELN
   481  KMETNKPRDL FIAKLLNKLK QQQQQQQQHS ENKRENSEDP EESWENLVSD EDFSALSLES
   541  ANVEDLEPVR NLFRKLQSTP KYQKLLKERQ QLPVFKHRDS IVETLKRHRV VVVAGETGSG
   601  KSTQVPHFLL EDLLLNEWEA SKCNIVCTQP RRISAVSLAN RVCDELGCEN GPGGRNSLCG
   661  YQIRMESRAC ESTRLLYCTT GVLLRKLQED GLLSNVSHVI VDEVHERSVQ SDFLLIILKE
   721  ILQKRSDLHL ILMSATVDSE KFSTYFTHCP ILRISGRSYP VEVFHLEDII EETGFVLEKD
   781  SEYCQKFLEE EEEVTINVTS KAGGIKKYQE YIPVQTGAHA DLNPFYQKYS SRTQHAILYM
   841  NPHKINLDLI LELLAYLDKS PQFRNIEGAV LIFLPGLAHI QQLYDLLSND RRFYSERYKV
   901  IALHSILSTQ DQAAAFTLPP PGVRKIVLAT NIAETGITIP DVVFVIDTGR TKENKYHESS
   961  QMSSLVETFV SKASALQRQG RAGRVRDGFC FRMYTRERFE GFMDYSVPEI LRVPLEELCL
  1021  HIMKCNLGSP EDFLSKALDP PQLQVISNAM NLLRKIGACE LNEPKLTPLG QHLAALPVNV
  1081  KIGKMLIFGA IFGCLDPVAT LAAVMTEKSP FTTPIGRKDE ADLAKSALAM ADSDHLTIYN
  1141  AYLGWKKARQ EGGYRSEITY CRRNFLNRTS LLTLEDVKQE LIKLVKAAGF SSSTTSTSWE
  1201  GNRASQTLSF QEIALLKAVL VAGLYDNVGK IIYTKSVDVT EKLACIVETA QGKAQVHPSS
  1261  VNRDLQTHGW LLYQEKIRYA RVYLRETTLI TPFPVLLFGG DIEVQHRERL LSIDGWIYFQ
  1321  APVKIAVIFK QLRVLIDSVL RKKLENPKMS LENDKILQII TELIKTENN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DHX29 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

22 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.53

gnomAD pLI

0

gnomAD missense Z

1.93

DepMap mean gene effect

-0.09

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• formation of translation preinitiation complex
• positive regulation of translational initiation
• ribosome assembly

Molecular functions

• ATP binding
• ATP hydrolysis activity
• cadherin binding
• helicase activity
• ribonucleoside triphosphate phosphatase activity
• ribosomal small subunit binding
• RNA binding
• RNA helicase activity
• translation activator activity
• translation initiation factor activity

Cellular components

• cytosolic small ribosomal subunit
• eukaryotic 43S preinitiation complex

Protein domainsUniProt · Pfam · InterPro

• Helicase, C-terminal domain-like
• DNA/RNA helicase, ATP-dependent, DEAH-box type, conserved site
• Helicase-associated domain
• DEAD/DEAH-box helicase domain
• DEAD-box helicase, OB fold
• Helicase superfamily 1/2, ATP-binding domain
• P-loop containing nucleoside triphosphate hydrolase
• Helicase associated domain (HA2), winged-helix domain
• RNA helicase, C-terminal domain
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain
• Helicase associated domain (HA2), winged-helix
• Oligonucleotide/oligosaccharide-binding (OB)-fold
• Helicase associated domain (HA2), ratchet-like
• C-terminal domain in RNA helicases
• ATP-dependent RNA helicase DHX29
• ATP-dependent RNA helicase DHX29, DSRM-like domain
• ATP-dependent RNA helicase DHX29-like, UBA domain
• ATP-dependent RNA helicase DHX29, DSRM-like
• ATP-dependent RNA helicase DHX29, UBA domain

KeywordsUniProt

• ATP-binding
• Coiled coil
• Cytoplasm
• Helicase
• Hydrolase
• Initiation factor
• Nucleotide-binding
• Phosphoprotein
• Protein biosynthesis

InteractionsUniProt · HPA

Protein binding partners of DHX29 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NPM1