DDX60

Protein identityUniProt · HPA

UniProt accession

Q8IY21

Gene

DDX60

Ensembl

ENSG00000137628

Chromosome

4

Canonical length

1712 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Intermediate filaments,Cytosol

OverviewNCBI Gene

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular procsses involving RNA binding and alteration of RNA secondary structure. This gene encodes a DEXD/H box RNA helicase that functions as an antiviral factor and promotes RIG-I-like receptor-mediated signaling. [provided by RefSeq, Apr 2017]

Canonical amino-acid sequenceUniProt

1712 residues, UniProt reviewed canonical sequence.

>Q8IY21|DDX60
     1  MERNVLTTFS QEMSQLILNE MPKAEYSSLF NDFVESEFFL IDGDSLLITC ICEISFKPGQ
    61  NLHFFYLVER YLVDLISKGG QFTIVFFKDA EYAYFNFPEL LSLRTALILH LQKNTTIDVR
   121  TTFSRCLSKE WGSFLEESYP YFLIVADEGL NDLQTQLFNF LIIHSWARKV NVVLSSGQES
   181  DVLCLYAYLL PSMYRHQIFS WKNKQNIKDA YTTLLNQLER FKLSALAPLF GSLKWNNITE
   241  EAHKTVSLLT QVWPEGSDIR RVFCVTSCSL SLRMYHRFLG NREPSSGQET EIQQVNSNCL
   301  TLQEMEDLCK LHCLTVVFLL HLPLSQRACA RVITSHWAED MKPLLQMKKW CEYFILRNIH
   361  TFEFWNLNLI HLSDLNDELL LKNIAFYYEN ENVKGLHLNL GDTIMKDYEY LWNTVSKLVR
   421  DFEVGQPFPL RTTKVCFLEK KPSPIKDSSN EMVPNLGFIP TSSFVVDKFA GDILKDLPFL
   481  KSDDPIVTSL VKQKEFDELV HWHSHKPLSD DYDRSRCQFD EKSRDPRVLR SVQKYHVFQR
   541  FYGNSLETVS SKIIVTQTIK SKKDFSGPKS KKAHETKAEI IARENKKRLF AREEQKEEQK
   601  WNALSFSIEE QLKENLHSGI KSLEDFLKSC KSSCVKLQVE MVGLTACLKA WKEHCRSEEG
   661  KTTKDLSIAV QVMKRIHSLM EKYSELLQED DRQLIARCLK YLGFDELASS LHPAQDAEND
   721  VKVKKRNKYS VGIGPARFQL QYMGHYLIRD ERKDPDPRVQ DFIPDTWQRE LLDVVDKNES
   781  AVIVAPTSSG KTYASYYCME KVLKESDDGV VVYVAPTKAL VNQVAATVQN RFTKNLPSGE
   841  VLCGVFTREY RHDALNCQVL ITVPACFEIL LLAPHRQNWV KKIRYVIFDE VHCLGGEIGA
   901  EIWEHLLVMI RCPFLALSAT ISNPEHLTEW LQSVKWYWKQ EDKIIENNTA SKRHVGRQAG
   961  FPKDYLQVKQ SYKVRLVLYG ERYNDLEKHV CSIKHGDIHF DHFHPCAALT TDHIERYGFP
  1021  PDLTLSPRES IQLYDAMFQI WKSWPRAQEL CPENFIHFNN KLVIKKMDAR KYEESLKAEL
  1081  TSWIKNGNVE QARMVLQNLS PEADLSPENM ITMFPLLVEK LRKMEKLPAL FFLFKLGAVE
  1141  NAAESVSTFL KKKQETKRPP KADKEAHVMA NKLRKVKKSI EKQKIIDEKS QKKTRNVDQS
  1201  LIHEAEHDNL VKCLEKNLEI PQDCTYADQK AVDTETLQKV FGRVKFERKG EELKALAERG
  1261  IGYHHSAMSF KEKQLVEILF RKGYLRVVTA TGTLALGVNM PCKSVVFAQN SVYLDALNYR
  1321  QMSGRAGRRG QDLMGDVYFF DIPFPKIGKL IKSNVPELRG HFPLSITLVL RLMLLASKGD
  1381  DPEDAKAKVL SVLKHSLLSF KQPRVMDMLK LYFLFSLQFL VKEGYLDQEG NPMGFAGLVS
  1441  HLHYHEPSNL VFVSFLVNGL FHDLCQPTRK GSKHFSQDVM EKLVLVLAHL FGRRYFPPKF
  1501  QDAHFEFYQS KVFLDDLPED FSDALDEYNM KIMEDFTTFL RIVSKLADMN QEYQLPLSKI
  1561  KFTGKECEDS QLVSHLMSCK EGRVAISPFV CLSGNFDDDL LRLETPNHVT LGTIGVNRSQ
  1621  APVLLSQKFD NRGRKMSLNA YALDFYKHGS LIGLVQDNRM NEGDAYYLLK DFALTIKSIS
  1681  VSLRELCENE DDNVVLAFEQ LSTTFWEKLN KV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against DDX60 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

39 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.59

gnomAD pLI

0

gnomAD missense Z

1.25

DepMap mean gene effect

0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• defense response to virus
• innate immune response
• positive regulation of MDA-5 signaling pathway
• positive regulation of RIG-I signaling pathway
• response to virus

Molecular functions

• ATP binding
• ATP hydrolysis activity
• double-stranded DNA binding
• double-stranded RNA binding
• RNA helicase activity
• single-stranded RNA binding

Cellular components

• cytoplasm
• cytosol
• intermediate filament cytoskeleton

Protein domainsUniProt · Pfam · InterPro

• Helicase, C-terminal domain-like
• DEAD/DEAH-box helicase domain
• Helicase superfamily 1/2, ATP-binding domain
• P-loop containing nucleoside triphosphate hydrolase
• SKI2 subfamily ATP-dependent RNA helicases
• ATP-dependent RNA helicase DDX60, PIN-like domain
• DDX60-like, winged helix domain
• DEAD/DEAH box helicase
• Helicase conserved C-terminal domain
• ATP-dependent RNA helicase DDX60, PIN-like domain
• DDX60-like, winged helix domain
• DDX60 TPR domain

KeywordsUniProt

• Antiviral defense
• ATP-binding
• Cytoplasm
• Helicase
• Hydrolase
• Immunity
• Innate immunity
• Nucleotide-binding
• RNA-binding

InteractionsUniProt · HPA

Protein binding partners of DDX60 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• EXOSC1
• EXOSC4
• RIGI
• IFIH1
• EIF3E