Seroatlas · Human Serome Atlas

CPSF1

Cleavage and polyadenylation specificity factor subunit 1

Also known as: CPSF1_HUMAN, CPSF160

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q10570
Gene: CPSF1
Ensembl: ENSG00000071894
Chromosome: 8
Canonical length: 1443 aa
Protein class: Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis. CPSF1 is the largest subunit of the CPSF complex (Murthy and Manley, 1995 [PubMed 7590244]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

1443 residues, UniProt reviewed canonical sequence.

>Q10570|CPSF1
     1  MYAVYKQAHP PTGLEFSMYC NFFNNSERNL VVAGTSQLYV YRLNRDAEAL TKNDRSTEGK
    61  AHREKLELAA SFSFFGNVMS MASVQLAGAK RDALLLSFKD AKLSVVEYDP GTHDLKTLSL
   121  HYFEEPELRD GFVQNVHTPR VRVDPDGRCA AMLVYGTRLV VLPFRRESLA EEHEGLVGEG
   181  QRSSFLPSYI IDVRALDEKL LNIIDLQFLH GYYEPTLLIL FEPNQTWPGR VAVRQDTCSI
   241  VAISLNITQK VHPVIWSLTS LPFDCTQALA VPKPIGGVVV FAVNSLLYLN QSVPPYGVAL
   301  NSLTTGTTAF PLRTQEGVRI TLDCAQATFI SYDKMVISLK GGEIYVLTLI TDGMRSVRAF
   361  HFDKAAASVL TTSMVTMEPG YLFLGSRLGN SLLLKYTEKL QEPPASAVRE AADKEEPPSK
   421  KKRVDATAGW SAAGKSVPQD EVDEIEVYGS EAQSGTQLAT YSFEVCDSIL NIGPCANAAV
   481  GEPAFLSEEF QNSPEPDLEI VVCSGHGKNG ALSVLQKSIR PQVVTTFELP GCYDMWTVIA
   541  PVRKEEEDNP KGEGTEQEPS TTPEADDDGR RHGFLILSRE DSTMILQTGQ EIMELDTSGF
   601  ATQGPTVFAG NIGDNRYIVQ VSPLGIRLLE GVNQLHFIPV DLGAPIVQCA VADPYVVIMS
   661  AEGHVTMFLL KSDSYGGRHH RLALHKPPLH HQSKVITLCL YRDLSGMFTT ESRLGGARDE
   721  LGGRSGPEAE GLGSETSPTV DDEEEMLYGD SGSLFSPSKE EARRSSQPPA DRDPAPFRAE
   781  PTHWCLLVRE NGTMEIYQLP DWRLVFLVKN FPVGQRVLVD SSFGQPTTQG EARREEATRQ
   841  GELPLVKEVL LVALGSRQSR PYLLVHVDQE LLIYEAFPHD SQLGQGNLKV RFKKVPHNIN
   901  FREKKPKPSK KKAEGGGAEE GAGARGRVAR FRYFEDIYGY SGVFICGPSP HWLLVTGRGA
   961  LRLHPMAIDG PVDSFAPFHN VNCPRGFLYF NRQGELRISV LPAYLSYDAP WPVRKIPLRC
  1021  TAHYVAYHVE SKVYAVATST NTPCARIPRM TGEEKEFETI ERDERYIHPQ QEAFSIQLIS
  1081  PVSWEAIPNA RIELQEWEHV TCMKTVSLRS EETVSGLKGY VAAGTCLMQG EEVTCRGRIL
  1141  IMDVIEVVPE PGQPLTKNKF KVLYEKEQKG PVTALCHCNG HLVSAIGQKI FLWSLRASEL
  1201  TGMAFIDTQL YIHQMISVKN FILAADVMKS ISLLRYQEES KTLSLVSRDA KPLEVYSVDF
  1261  MVDNAQLGFL VSDRDRNLMV YMYLPEAKES FGGMRLLRRA DFHVGAHVNT FWRTPCRGAT
  1321  EGLSKKSVVW ENKHITWFAT LDGGIGLLLP MQEKTYRRLL MLQNALTTML PHHAGLNPRA
  1381  FRMLHVDRRT LQNAVRNVLD GELLNRYLYL STMERSELAK KIGTTPDIIL DDLLETDRVT
  1441  AHF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CPSF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.29
Highest tissue expression: 128 nTPM

Expression across tissuesHPA

Tissue

testis: 128 nTPM
pancreas: 86 nTPM
pituitary gland: 78 nTPM
liver: 75 nTPM
cerebellum: 69 nTPM
spleen: 68 nTPM

Single-cell type

sertoli cells: 66 nCPM
late spermatids: 54 nCPM
early primary spermatocytes: 31 nCPM
enteric transient amplifying cells: 30 nCPM
foveolar cells: 28 nCPM
differentiating spermatogonia: 25 nCPM

Immune cell

T-reg: 6.8 nTPM
non-classical monocyte: 6.2 nTPM
intermediate monocyte: 6.1 nTPM
myeloid DC: 5.7 nTPM
memory CD4 T-cell: 5.2 nTPM
classical monocyte: 5.1 nTPM

Brain region

cerebral cortex: 20 nTPM
pons: 17 nTPM
medulla oblongata: 16 nTPM
basal ganglia: 16 nTPM
choroid plexus: 15 nTPM
amygdala: 15 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CPSF1.

Disease | AllUniProt

Conditions CPSF1 is implicated in, by any mechanism.

Myopia 27, autosomal dominant (MYP27) MIM:618827

Disease | GeneticClinVar

8 pathogenic / likely-pathogenic of 318 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Myopia 27

Disease | ImmuneIEDB

Conditions an epitope on CPSF1 was assayed in.

melanoma T cell
skin melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.54
gnomAD pLI: 0
gnomAD missense Z: 2.8
DepMap mean gene effect: -1.18
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 15% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

mRNA processing
co-transcriptional RNA 3'-end processing, cleavage and polyadenylation pathway

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CPSF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CPSF1 as an antibody target. Whether an autoantibody or antibody against CPSF1 could matter depends on whether native CPSF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CPSF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CPSF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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