Seroatlas · Human Serome Atlas

CPSF4

Cleavage and polyadenylation specificity factor subunit 4

Also known as: CPSF30, CPSF4_HUMAN, NAR

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O95639
Gene: CPSF4
Ensembl: ENSG00000160917
Chromosome: 7
Canonical length: 269 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Vesicles

OverviewNCBI Gene

Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

269 residues, UniProt reviewed canonical sequence.

>O95639|CPSF4
     1  MQEIIASVDH IKFDLEIAVE QQLGAQPLPF PGMDKSGAAV CEFFLKAACG KGGMCPFRHI
    61  SGEKTVVCKH WLRGLCKKGD QCEFLHEYDM TKMPECYFYS KFGECSNKEC PFLHIDPESK
   121  IKDCPWYDRG FCKHGPLCRH RHTRRVICVN YLVGFCPEGP SCKFMHPRFE LPMGTTEQPP
   181  LPQQTQPPAK QSNNPPLQRS SSLIQLTSQN SSPNQQRTPQ VIGVMQSQNS SAGNRGPRPL
   241  EQVTCYKCGE KGHYANRCTK GHLAFLSGQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CPSF4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.5
Highest tissue expression: 33 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 33 nTPM
testis: 30 nTPM
cerebellum: 28 nTPM
cerebral cortex: 28 nTPM
pancreas: 28 nTPM
amygdala: 26 nTPM

Single-cell type

cytotrophoblasts: 74 nCPM
sertoli cells: 65 nCPM
migrating cytotrophoblasts: 61 nCPM
syncytiotrophoblasts: 58 nCPM
esophageal apical cells: 55 nCPM
extravillous trophoblasts: 38 nCPM

Immune cell

naive B-cell: 8.5 nTPM
memory B-cell: 8.2 nTPM
T-reg: 8.2 nTPM
naive CD4 T-cell: 6.3 nTPM
memory CD8 T-cell: 6.1 nTPM
naive CD8 T-cell: 5.6 nTPM

Brain region

medulla oblongata: 41 nTPM
thalamus: 34 nTPM
midbrain: 33 nTPM
amygdala: 32 nTPM
hypothalamus: 31 nTPM
pons: 31 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.56
gnomAD pLI: 0.27
gnomAD missense Z: 3.29
DepMap mean gene effect: -1.46
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

mRNA processing

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CPSF4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CPSF4 as an antibody target. Whether an autoantibody or antibody against CPSF4 could matter depends on whether native CPSF4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CPSF4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CPSF4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...