Seroatlas · Human Serome Atlas

CHFR

E3 ubiquitin-protein ligase CHFR

Also known as: CHFR_HUMAN, FLJ10796, RNF196

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96EP1
Gene: CHFR
Ensembl: ENSG00000072609
Chromosome: 12
Canonical length: 664 aa
Protein class: Cancer-related genes, Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nuclear bodies,Microtubules

OverviewNCBI Gene

This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]

Canonical amino-acid sequenceUniProt

664 residues, UniProt reviewed canonical sequence.

>Q96EP1|CHFR
     1  MERPEEGKQS PPPQPWGRLL RLGAEEGEPH VLLRKREWTI GRRRGCDLSF PSNKLVSGDH
    61  CRIVVDEKSG QVTLEDTSTS GTVINKLKVV KKQTCPLQTG DVIYLVYRKN EPEHNVAYLY
   121  ESLSEKQGMT QESFEANKEN VFHGTKDTSG AGAGRGADPR VPPSSPATQV CFEEPQPSTS
   181  TSDLFPTASA SSTEPSPAGR ERSSSCGSGG GGISPKGSGP SVASDEVSSF ASALPDRKTA
   241  SFSSLEPQDQ EDLEPVKKKM RGDGDLDLNG QLLVAQPRRN AQTVHEDVRA AAGKPDKMEE
   301  TLTCIICQDL LHDCVSLQPC MHTFCAACYS GWMERSSLCP TCRCPVERIC KNHILNNLVE
   361  AYLIQHPDKS RSEEDVQSMD ARNKITQDML QPKVRRSFSD EEGSSEDLLE LSDVDSESSD
   421  ISQPYVVCRQ CPEYRRQAAQ PPHCPAPEGE PGAPQALGDA PSTSVSLTTA VQDYVCPLQG
   481  SHALCTCCFQ PMPDRRAERE QDPRVAPQQC AVCLQPFCHL YWGCTRTGCY GCLAPFCELN
   541  LGDKCLDGVL NNNSYESDIL KNYLATRGLT WKNMLTESLV ALQRGVFLLS DYRVTGDTVL
   601  CYCCGLRSFR ELTYQYRQNI PASELPVAVT SRPDCYWGRN CRTQVKAHHA MKFNHICEQT
   661  RFKN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CHFR can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.46
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

thymus: 23 nTPM
bone marrow: 21 nTPM
spleen: 20 nTPM
lymph node: 20 nTPM
esophagus: 19 nTPM
placenta: 17 nTPM

Single-cell type

early spermatids: 191 nCPM
neutrophils: 144 nCPM
late spermatids: 135 nCPM
retinal ganglion cells: 124 nCPM
rod photoreceptor cells: 120 nCPM
retinal horizontal cells: 96 nCPM

Immune cell

basophil: 39 nTPM
eosinophil: 36 nTPM
neutrophil: 35 nTPM
non-classical monocyte: 32 nTPM
intermediate monocyte: 30 nTPM
classical monocyte: 26 nTPM

Brain region

thalamus: 24 nTPM
white matter: 24 nTPM
cerebral cortex: 23 nTPM
medulla oblongata: 22 nTPM
pons: 22 nTPM
amygdala: 21 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.55
gnomAD pLI: 0
gnomAD missense Z: 1.21
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Forkhead-associated (FHA) domain
Zinc finger, RING-type
SMAD/FHA domain superfamily
Zinc finger, RING/FYVE/PHD-type
Zinc finger, RING-type, conserved site
FHA domain
Zinc finger, C3HC4 type (RING finger)
E3 ubiquitin-protein ligase CHFR, cysteine rich domain with multizinc binding
E3 ubiquitin-protein ligase CHFR
Cysteine rich domain with multizinc binding regions

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CHFR in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CHFR as an antibody target. Whether an autoantibody or antibody against CHFR could matter depends on whether native CHFR is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CHFR is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CHFR as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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