Seroatlas · Human Serome Atlas

CCDC66

Coiled-coil domain-containing protein 66

Also known as: CCD66_HUMAN, DKFZp686C0433

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A2RUB6
Gene: CCDC66
Ensembl: ENSG00000180376
Chromosome: 3
Canonical length: 948 aa
Protein class: Predicted intracellular proteins
Subcellular location: Vesicles,Plasma membrane,Cell Junctions,Midbody ring,Primary cilium,Centriolar satellite,Cytosol,Perinuclear theca,End piece
Quaternary structure: Homodimer

OverviewNCBI Gene

Enables microtubule binding activity. Involved in several processes, including ciliary transition zone assembly; microtubule cytoskeleton organization; and mitotic metaphase chromosome alignment. Located in several cellular components, including Flemming body; cytoskeleton; and photoreceptor inner segment. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

948 residues, UniProt reviewed canonical sequence.

>A2RUB6|CCDC66
     1  MNLGDGLKLE TELLDGKTKL ILSPYEHKSK ISVKMGNKAK IAKCPLRTKT GHILKSTQDT
    61  CIGSEKLLQK KPVGSETSQA KGEKNGMTFS STKDLCKQCI DKDCLHIQKE ISPATPNMQK
   121  TRNTVNTSLV GKQKPHKKHI TAENMKSSLV CLTQDQLQQI LMTVNQGNRS LSLTENGKEA
   181  KSQYSLYLNS ISNQPKDENI MGLFKKTEMV SSVPAENKSV LNEHQETSKQ CEQKIAIENE
   241  WKPADIFSTL GERECDRSSL EAKKAQWRKE LDEQVALKKK EKEVSEKWND PWKKSESDKI
   301  IWEKHQILDQ SRETVLLEHP FSAVKQELQR KWIEELNKQI EDDRQRKIEE KIIYSKGEEH
   361  DRWAMHFDSL KSYPGSQSQL FSQSTHKQPE YFCVSPDTQE LADVSSVCTP TTGSQVEPSE
   421  EEHIAKPIKD VVMANSKKTN FLRSMTALLD PAQIEERDRR RQKQLEHQKA ITAQVEEKRR
   481  KKQLEEEQRK KEEQEEELRL AQEREEMQKQ YEEDILKQKQ KEEIMTLKTN ELFQTMQRAQ
   541  ELAQRLKQEQ RIRELAQKGH DTSRLIKNLG VDTIQMEYNA SNISNSRHDS DEISGKMNTY
   601  MNSTTSKKDT GVQTDDLNIG IFTNAESHCG SLMERDITNC SSPEISAELI GQFSTKKNKQ
   661  ELTQDKGASL EKENNRCNDQ CNQFTRIEKQ TKHMKKYPKR PDWNINKPPK RYIPASEKYP
   721  KQLQKQREEK KVRRQMELLH LVEKNNPGHL SQNRGISPEI FHSSHQETES KLRWHLVKKE
   781  EEPLNIHSFS KERSPSSPVP VVKNRTQQTQ NTLHLPLKNS SYERENLISG SNQTELSSGI
   841  SESSHFIPYV RTNEIYYLDP DAPLSGPSTQ DPQYQNSQDC GQKRQLFDSD CVRDPLLNPN
   901  MVKNRDRQQA ILKGLSELRQ GLLQKQKELE SSLLPLAENQ EESFGSSF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CCDC66 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.63
Highest tissue expression: 7.3 nTPM

Expression across tissuesHPA

Tissue

retina: 7.3 nTPM
choroid plexus: 6 nTPM
fallopian tube: 5.4 nTPM
parathyroid gland: 5.1 nTPM
epididymis: 5 nTPM
breast: 4.9 nTPM

Single-cell type

early spermatids: 237 nCPM
lactotrophs: 203 nCPM
ependymal cells: 201 nCPM
thyrotrophs: 193 nCPM
somatotrophs: 183 nCPM
choroid plexus epithelial cells: 173 nCPM

Immune cell

T-reg: 15 nTPM
basophil: 10 nTPM
naive CD4 T-cell: 7.9 nTPM
memory CD4 T-cell: 6.9 nTPM
naive CD8 T-cell: 5.7 nTPM
MAIT T-cell: 5.4 nTPM

Brain region

hypothalamus: 3.5 nTPM
choroid plexus: 3.3 nTPM
medulla oblongata: 2.7 nTPM
pons: 2.5 nTPM
spinal cord: 2.5 nTPM
cerebral cortex: 2.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.22
gnomAD pLI: 0
gnomAD missense Z: -1.04
DepMap mean gene effect: -0.03
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Coiled-coil domain-containing protein 66
CCDC66 domain
Coiled-coil domain-containing protein 66

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CCDC66 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CCDC66 as an antibody target. Whether an autoantibody or antibody against CCDC66 could matter depends on whether native CCDC66 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CCDC66 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CCDC66 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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