Seroatlas · Human Serome Atlas

CASP4

Caspase-4

Also known as: CASP4_HUMAN, ICE(rel)II, ICH-2, TX

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P49662
Gene
CASP4
Ensembl
ENSG00000196954
Chromosome
11
Canonical length
377 aa
Protein class
Cancer-related genes, Enzymes, Predicted intracellular proteins
Subcellular location
Plasma membrane,Cytosol
Secretome location
Intracellular and membrane
Quaternary structure
Homooligomer

OverviewNCBI Gene

This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

377 residues, UniProt reviewed canonical sequence.

>P49662|CASP4
     1  MAEGNHRKKP LKVLESLGKD FLTGVLDNLV EQNVLNWKEE EKKKYYDAKT EDKVRVMADS
    61  MQEKQRMAGQ MLLQTFFNID QISPNKKAHP NMEAGPPESG ESTDALKLCP HEEFLRLCKE
   121  RAEEIYPIKE RNNRTRLALI ICNTEFDHLP PRNGADFDIT GMKELLEGLD YSVDVEENLT
   181  ARDMESALRA FATRPEHKSS DSTFLVLMSH GILEGICGTV HDEKKPDVLL YDTIFQIFNN
   241  RNCLSLKDKP KVIIVQACRG ANRGELWVRD SPASLEVASS QSSENLEEDA VYKTHVEKDF
   301  IAFCSSTPHN VSWRDSTMGS IFITQLITCF QKYSWCCHLE EVFRKVQQSF ETPRAKAQMP
   361  TIERLSMTRY FYLFPGN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CASP4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Secreted
Secreted
Yes
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
77 nTPM

Expression across tissuesHPA

Tissue

  • tonsil: 77 nTPM
  • lymph node: 75 nTPM
  • urinary bladder: 71 nTPM
  • spleen: 70 nTPM
  • pancreas: 66 nTPM
  • placenta: 66 nTPM

Single-cell type

  • neutrophils: 662 nCPM
  • esophageal apical cells: 510 nCPM
  • platelets: 230 nCPM
  • pancreatic acinar cells: 206 nCPM
  • monocytes: 166 nCPM
  • cytotrophoblasts: 160 nCPM

Immune cell

  • neutrophil: 670 nTPM
  • total PBMC: 411 nTPM
  • intermediate monocyte: 313 nTPM
  • classical monocyte: 292 nTPM
  • T-reg: 259 nTPM
  • non-classical monocyte: 257 nTPM

Brain region

  • medulla oblongata: 11 nTPM
  • thalamus: 10 nTPM
  • cerebral cortex: 9.4 nTPM
  • pons: 9.3 nTPM
  • white matter: 9.3 nTPM
  • choroid plexus: 8.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.17
gnomAD pLI
0
gnomAD missense Z
0.18
DepMap mean gene effect
0.17
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CASP4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CASP4 as an antibody target. Whether an autoantibody or antibody against CASP4 could matter depends on whether native CASP4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CASP4 is annotated as secreted, so native CASP4 circulates and is directly accessible to antibodies. Secreted and cell-surface proteins are the autoantibody targets most likely to act like drugs, blocking or depleting the native protein.

Annotation status

The present source text does not explicitly label CASP4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CASP4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...