Seroatlas · Human Serome Atlas

TMEM214

Transmembrane protein 214

Also known as: FLJ20254, TM214_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6NUQ4
Gene: TMEM214
Ensembl: ENSG00000119777
Chromosome: 2
Canonical length: 689 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Endoplasmic reticulum,Golgi apparatus,Cytosol

OverviewNCBI Gene

Predicted to be involved in apoptotic process. Located in several cellular components, including Golgi apparatus; cytoplasmic microtubule; and endoplasmic reticulum. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

689 residues, UniProt reviewed canonical sequence.

>Q6NUQ4|TMEM214
     1  MATKTAGVGR WEVVKKGRRP GVGAGAGGRG GGRNRRALGE ANGVWKYDLT PAIQTTSTLY
    61  ERGFENIMKR QNKEQVPPPA VEPKKPGNKK QPKKVATPPN QNQKQGRFRS LEEALKALDV
   121  ADLQKELDKS QSVFSGNPSI WLKDLASYLN YKLQAPLSEP TLSQHTHDYP YSLVSRELRG
   181  IIRGLLAKAA GSLELFFDHC LFTMLQELDK TPGESLHGYR ICIQAILQDK PKIATANLGK
   241  FLELLRSHQS RPAKCLTIMW ALGQAGFANL TEGLKVWLGI MLPVLGIKSL SPFAITYLDR
   301  LLLMHPNLTK GFGMIGPKDF FPLLDFAYMP NNSLTPSLQE QLCQLYPRLK VLAFGAKPDS
   361  TLHTYFPSFL SRATPSCPPE MKKELLSSLT ECLTVDPLSA SVWRQLYPKH LSQSSLLLEH
   421  LLSSWEQIPK KVQKSLQETI QSLKLTNQEL LRKGSSNNQD VVTCDMACKG LLQQVQGPRL
   481  PWTRLLLLLL VFAVGFLCHD LRSHSSFQAS LTGRLLRSSG FLPASQQACA KLYSYSLQGY
   541  SWLGETLPLW GSHLLTVVRP SLQLAWAHTN ATVSFLSAHC ASHLAWFGDS LTSLSQRLQI
   601  QLPDSVNQLL RYLRELPLLF HQNVLLPLWH LLLEALAWAQ EHCHEACRGE VTWDCMKTQL
   661  SEAVHWTWLC LQDITVAFLD WALALISQQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMEM214 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.38
Highest tissue expression: 79 nTPM

Expression across tissuesHPA

Tissue

salivary gland: 79 nTPM
pancreas: 76 nTPM
epididymis: 63 nTPM
pituitary gland: 59 nTPM
blood vessel: 56 nTPM
parathyroid gland: 53 nTPM

Single-cell type

syncytiotrophoblasts: 85 nCPM
extravillous trophoblasts: 80 nCPM
plasma cells: 64 nCPM
hepatic stellate cells: 58 nCPM
salivary acinar cells: 56 nCPM
epididymal principal cells: 52 nCPM

Immune cell

basophil: 5.7 nTPM
NK-cell: 5.6 nTPM
MAIT T-cell: 5 nTPM
plasmacytoid DC: 5 nTPM
gdT-cell: 3.9 nTPM
intermediate monocyte: 3.8 nTPM

Brain region

choroid plexus: 56 nTPM
medulla oblongata: 36 nTPM
midbrain: 35 nTPM
pons: 34 nTPM
thalamus: 33 nTPM
hypothalamus: 31 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.8
gnomAD pLI: 0
gnomAD missense Z: 0.73
DepMap mean gene effect: -0.01
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

apoptotic process

Cellular components

Protein domainsUniProt · Pfam · InterPro

Transmembrane protein 214
TMEM214, C-terminal, caspase 4 activator

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMEM214 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

CASP4

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMEM214 as an antibody target. Whether an autoantibody or antibody against TMEM214 could matter depends on whether native TMEM214 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMEM214 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMEM214 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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