CTSG

Protein identityUniProt · HPA

UniProt accession

P08311

Gene

CTSG

Ensembl

ENSG00000100448

Chromosome

14

Canonical length

255 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Secretome location

Intracellular and membrane

OverviewNCBI Gene

The protein encoded by this gene, a member of the peptidase S1 protein family, is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation. In addition, the encoded protein is antimicrobial, with bacteriocidal activity against S. aureus and N. gonorrhoeae. Transcript variants utilizing alternative polyadenylation signals exist for this gene. [provided by RefSeq, Sep 2014]

Canonical amino-acid sequenceUniProt

255 residues, UniProt reviewed canonical sequence.

>P08311|CTSG
     1  MQPLLLLLAF LLPTGAEAGE IIGGRESRPH SRPYMAYLQI QSPAGQSRCG GFLVREDFVL
    61  TAAHCWGSNI NVTLGAHNIQ RRENTQQHIT ARRAIRHPQY NQRTIQNDIM LLQLSRRVRR
   121  NRNVNPVALP RAQEGLRPGT LCTVAGWGRV SMRRGTDTLR EVQLRVQRDR QCLRIFGSYD
   181  PRRQICVGDR RERKAAFKGD SGGPLLCNNV AHGIVSYGKS SGVPPEVFTR VSSFLPWIRT
   241  TMRSFKLLDQ METPL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CTSG can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

5,049 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CTSG.

Disease | ImmuneIEDB

Conditions an epitope on CTSG was assayed in.

• acute myeloid leukemia T cell

ReferencesPubMed · IEDB

Publications for CTSG from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.32

gnomAD pLI

0

gnomAD missense Z

0.82

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• angiotensin maturation
• antibacterial humoral response
• cellular response to lipopolysaccharide
• cytokine-mediated signaling pathway
• defense response to fungus
• defense response to Gram-negative bacterium
• defense response to Gram-positive bacterium
• extracellular matrix disassembly
• immune response
• monocyte chemotaxis
• negative regulation of T cell activation
• neutrophil activation
• neutrophil-mediated killing of gram-positive bacterium
• platelet activation
• positive regulation of immune response
• positive regulation of platelet aggregation
• protein maturation
• protein processing
• proteolysis
• purinergic nucleotide receptor signaling pathway
• biofilm matrix disassembly

Molecular functions

• caspase binding
• heparin binding
• peptidase activity
• receptor ligand activity
• serine-type endopeptidase activity
• serine-type peptidase activity

Cellular components

• azurophil granule lumen
• cytoplasmic stress granule
• cytosol
• extracellular exosome
• extracellular matrix
• extracellular region
• extracellular space
• lysosome
• membrane
• nucleus
• plasma membrane
• secretory granule

Protein domainsUniProt · Pfam · InterPro

• Serine proteases, trypsin domain
• Peptidase S1A, chymotrypsin family
• Peptidase S1, PA clan
• Serine proteases, trypsin family, histidine active site
• Serine proteases, trypsin family, serine active site
• Trypsin

KeywordsUniProt

• Antibiotic
• Antimicrobial
• Cell membrane
• Chemotaxis
• Cytoplasm
• Disulfide bond
• Glycoprotein
• Hydrolase
• Lysosome
• Membrane
• Nucleus
• Protease
• Secreted
• Serine protease
• Signal
• Zymogen

InteractionsUniProt · HPA

Protein binding partners of CTSG in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CASP4