Seroatlas · Human Serome Atlas

CARD9

Caspase recruitment domain-containing protein 9

Also known as: CARD9_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H257
Gene
CARD9
Ensembl
ENSG00000187796
Chromosome
9
Canonical length
536 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

536 residues, UniProt reviewed canonical sequence.

>Q9H257|CARD9
     1  MSDYENDDEC WSVLEGFRVT LTSVIDPSRI TPYLRQCKVL NPDDEEQVLS DPNLVIRKRK
    61  VGVLLDILQR TGHKGYVAFL ESLELYYPQL YKKVTGKEPA RVFSMIIDAS GESGLTQLLM
   121  TEVMKLQKKV QDLTALLSSK DDFIKELRVK DSLLRKHQER VQRLKEECEA GSRELKRCKE
   181  ENYDLAMRLA HQSEEKGAAL MRNRDLQLEI DQLKHSLMKA EDDCKVERKH TLKLRHAMEQ
   241  RPSQELLWEL QQEKALLQAR VQELEASVQE GKLDRSSPYI QVLEEDWRQA LRDHQEQANT
   301  IFSLRKDLRQ GEARRLRCME EKEMFELQCL ALRKDSKMYK DRIEAILLQM EEVAIERDQA
   361  IATREELHAQ HARGLQEKDA LRKQVRELGE KADELQLQVF QCEAQLLAVE GRLRRQQLET
   421  LVLSSDLEDG SPRRSQELSL PQDLEDTQLS DKGCLAGGGS PKQPFAALHQ EQVLRNPHDA
   481  GLSSGEPPEK ERRRLKESFE NYRRKRALRK MQKGWRQGEE DRENTTGSDN TDTEGS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CARD9 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.46
Highest tissue expression
8.1 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 8.1 nTPM
  • bone marrow: 6.8 nTPM
  • appendix: 2.8 nTPM
  • lung: 2.5 nTPM
  • spinal cord: 1.9 nTPM
  • cervix: 1.5 nTPM

Single-cell type

  • microglia: 23 nCPM
  • renal collecting duct principal cells: 4.8 nCPM
  • oocytes: 4.6 nCPM
  • astrocytes: 2.1 nCPM
  • podocytes: 2.1 nCPM
  • brain inhibitory neurons: 1.7 nCPM

Immune cell

  • intermediate monocyte: 56 nTPM
  • non-classical monocyte: 49 nTPM
  • classical monocyte: 33 nTPM
  • myeloid DC: 25 nTPM
  • total PBMC: 14 nTPM
  • eosinophil: 10 nTPM

Brain region

  • cerebellum: 5.5 nTPM
  • medulla oblongata: 3.7 nTPM
  • white matter: 3.4 nTPM
  • spinal cord: 2.6 nTPM
  • thalamus: 2.6 nTPM
  • pons: 2.4 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CARD9.

Disease | AllUniProt

Conditions CARD9 is implicated in, by any mechanism.

  • Immunodeficiency 103, susceptibility to fungal infections (IMD103) MIM:212050

Disease | GeneticClinVar

24 pathogenic / likely-pathogenic of 627 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.13
gnomAD pLI
0
gnomAD missense Z
0.1
DepMap mean gene effect
-0.06
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CARD9 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CARD9 as an antibody target. Whether an autoantibody or antibody against CARD9 could matter depends on whether native CARD9 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CARD9 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CARD9 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CARD9. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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