RASGRF1

Protein identityUniProt · HPA

UniProt accession

Q13972

Gene

RASGRF1

Ensembl

ENSG00000058335

Chromosome

15

Canonical length

1273 aa

Protein class

Predicted intracellular proteins, RAS pathway related proteins

Subcellular location

Golgi apparatus

Quaternary structure

Homooligomer

OverviewNCBI Gene

The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) similar to the Saccharomyces cerevisiae CDC25 gene product. Functional analysis has demonstrated that this protein stimulates the dissociation of GDP from RAS protein. The studies of the similar gene in mouse suggested that the Ras-GEF activity of this protein in brain can be activated by Ca2+ influx, muscarinic receptors, and G protein beta-gamma subunit. Mouse studies also indicated that the Ras-GEF signaling pathway mediated by this protein may be important for long-term memory. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Mar 2009]

Canonical amino-acid sequenceUniProt

1273 residues, UniProt reviewed canonical sequence.

>Q13972|RASGRF1
     1  MQKGIRLNDG HVASLGLLAR KDGTRKGYLS KRSSDNTKWQ TKWFALLQNL LFYFESDSSS
    61  RPSGLYLLEG CVCDRAPSPK PALSAKEPLE KQHYFTVNFS HENQKALELR TEDAKDCDEW
   121  VAAIAHASYR TLATEHEALM QKYLHLLQIV ETEKTVAKQL RQQIEDGEIE IERLKAEITS
   181  LLKDNERIQS TQTVAPNDED SDIKKIKKVQ SFLRGWLCRR KWKTIIQDYI RSPHADSMRK
   241  RNQVVFSMLE AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH
   301  QIFYQGLKAR ISSWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC KQNRDFDKLL
   361  KHYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP HEHVERNSLD YAKSKLEELS
   421  RIMHDEVSET ENIRKNLAIE RMIIEGCEIL LDTSQTFVRQ GSLIQVPMSE KGKITRGRLG
   481  SLSLKKEGER QCFLFSKHLI ICTRGSGGKL HLTKNGVISL IDCTLLEEPE STEEEAKGSG
   541  QDIDHLDFKI GVEPKDSPPF TVILVASSRQ EKAAWTSDIS QCVDNIRCNG LMMNAFEENS
   601  KVTVPQMIKR TREGTREAEM SRSDASLYCD DVDIRFSKTM NSCKVLQIRY ASVERLLERL
   661  TDLRFLSIDF LNTFLHSYRV FTTAIVVLDK LITIYKKPIS AIPARWLRSL ELLFASGQNN
   721  KLLYGEPPKS PRATRKFSSP PPLSITKTSS PSRRRKLSLN IPIITGGKAL DLAALSCNSN
   781  GYTSMYSAMS PFSKATLDTS KLYVSSSFTN KIPDEGDTTP EKPEDPSALS KQSSEVSMRE
   841  ESDIDQNQSD DGDTETSPTK SPTTPKSVKN KNSSEFPLFS YNNGVVMTSC RELDNNRSAL
   901  SAASAFAIAT AGANEGTPNK EKYRRMSLAS AGFPPDQRNG DKEFVIRRAA TNRVLNVLRH
   961  WVSKHSQDFE TNDELKCKVI GFLEEVMHDP ELLTQERKAA ANIIRTLTQE DPGDNQITLE
  1021  EITQMAEGVK AEPFENHSAL EIAEQLTLLD HLVFKKIPYE EFFGQGWMKL EKNERTPYIM
  1081  KTTKHFNDIS NLIASEIIRN EDINARVSAI EKWVAVADIC RCLHNYNAVL EITSSMNRSA
  1141  IFRLKKTWLK VSKQTKALID KLQKLVSSEG RFKNLREALK NCDPPCVPYL GMYLTDLAFI
  1201  EEGTPNYTED GLVNFSKMRM ISHIIREIRQ FQQTAYKIEH QAKVTQYLLD QSFVMDEESL
  1261  YESSLRIEPK LPT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RASGRF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.38

Highest tissue expression

52 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RASGRF1.

Disease | ImmuneIEDB

Conditions an epitope on RASGRF1 was assayed in.

• chronic lymphocytic leukemia T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.21

gnomAD pLI

1

gnomAD missense Z

4.21

DepMap mean gene effect

0.06

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• long-term memory
• negative regulation of MAPK cascade
• neuron projection development
• positive regulation of MAPK cascade
• positive regulation of Rac protein signal transduction
• positive regulation of Ras protein signal transduction
• Ras protein signal transduction
• regulation of neuronal synaptic plasticity
• regulation of Ras protein signal transduction
• regulation of synaptic plasticity
• response to endoplasmic reticulum stress
• signal transduction
• type B pancreatic cell proliferation

Molecular functions

• glutamate receptor binding
• guanyl-nucleotide exchange factor activity

Cellular components

• cytosol
• growth cone
• neuron projection
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Dbl homology domain
• Ras-like guanine nucleotide exchange factor, N-terminal
• Guanine-nucleotide dissociation stimulator, CDC24, conserved site
• Pleckstrin homology domain
• Ras guanine-nucleotide exchange factors catalytic domain
• Ras-like guanine nucleotide exchange factor
• PH-like domain superfamily
• Ras guanine-nucleotide exchange factor, conserved site
• Ras guanine nucleotide exchange factor domain superfamily
• Dbl homology (DH) domain superfamily
• Ras guanine-nucleotide exchange factor, catalytic domain superfamily
• PH domain
• RasGEF domain
• RasGEF N-terminal motif
• RhoGEF domain

KeywordsUniProt

• Guanine-nucleotide releasing factor
• Phosphoprotein
• Repeat
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of RASGRF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• USP8
• CARD9