Seroatlas · Human Serome Atlas

CARD8

Caspase recruitment domain-containing protein 8

Also known as: CARD8_HUMAN, CARDINAL, Dakar, KIAA0955, NDPP, TUCAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y2G2
Gene
CARD8
Ensembl
ENSG00000105483
Chromosome
19
Canonical length
537 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm
Quaternary structure
Homodimer

OverviewNCBI Gene

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Canonical amino-acid sequenceUniProt

537 residues, UniProt reviewed canonical sequence.

>Q9Y2G2|CARD8
     1  MEKKECPEKS SSSEEELPRR DSGSSRNIDA SKLIRLQGSR KLLVDNSIRE LQYTKTGIFF
    61  QAEACVTNDT VYRELPCVSE TLCDISHFFQ EDDETEAEPL LFRAVPECQL SGGDIPSVSE
   121  EQESSEGQDS GDICSEENQI VSSYASKVCF EIEEDYKNRQ FLGPEGNVDV ELIDKSTNRY
   181  SVWFPTAGWY LWSATGLGFL VRDEVTVTIA FGSWSQHLAL DLQHHEQWLV GGPLFDVTAE
   241  PEEAVAEIHL PHFISLQAGE VDVSWFLVAH FKNEGMVLEH PARVEPFYAV LESPSFSLMG
   301  ILLRIASGTR LSIPITSNTL IYYHPHPEDI KFHLYLVPSD ALLTKAIDDE EDRFHGVRLQ
   361  TSPPMEPLNF GSSYIVSNSA NLKVMPKELK LSYRSPGEIQ HFSKFYAGQM KEPIQLEITE
   421  KRHGTLVWDT EVKPVDLQLV AASAPPPFSG AAFVKENHRQ LQARMGDLKG VLDDLQDNEV
   481  LTENEKELVE QEKTRQSKNE ALLSMVEKKG DLALDVLFRS ISERDPYLVS YLRQQNL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against CARD8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.39
Highest tissue expression
37 nTPM

Expression across tissuesHPA

Tissue

  • spleen: 37 nTPM
  • lymph node: 36 nTPM
  • tonsil: 35 nTPM
  • thymus: 29 nTPM
  • appendix: 27 nTPM
  • adipose tissue: 26 nTPM

Single-cell type

  • neutrophils: 385 nCPM
  • neutrophil progenitors: 157 nCPM
  • microglia: 149 nCPM
  • vascular endothelial cells: 136 nCPM
  • platelets: 132 nCPM
  • hematopoietic stem cells: 127 nCPM

Immune cell

  • neutrophil: 168 nTPM
  • basophil: 105 nTPM
  • eosinophil: 77 nTPM
  • non-classical monocyte: 69 nTPM
  • intermediate monocyte: 64 nTPM
  • classical monocyte: 46 nTPM

Brain region

  • thalamus: 23 nTPM
  • white matter: 21 nTPM
  • choroid plexus: 21 nTPM
  • medulla oblongata: 21 nTPM
  • spinal cord: 19 nTPM
  • pons: 19 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about CARD8.

Disease | AllUniProt

Conditions CARD8 is implicated in, by any mechanism.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 495 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Inflammatory bowel disease 30

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.88
gnomAD pLI
0
gnomAD missense Z
0.92
DepMap mean gene effect
-0.08
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of CARD8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads CARD8 as an antibody target. Whether an autoantibody or antibody against CARD8 could matter depends on whether native CARD8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

CARD8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label CARD8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/CARD8. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

© 2026 · Seroatlas, Discover Oregon LLC · Terms of use

Loading the interactive Seroatlas protein explorer...