Seroatlas · Human Serome Atlas

BUB3

Mitotic checkpoint protein BUB3

Also known as: BUB3_HUMAN, BUB3L

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O43684
Gene: BUB3
Ensembl: ENSG00000154473
Chromosome: 10
Canonical length: 328 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

328 residues, UniProt reviewed canonical sequence.

>O43684|BUB3
     1  MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR LKYQHTGAVL
    61  DCAFYDPTHA WSGGLDHQLK MHDLNTDQEN LVGTHDAPIR CVEYCPEVNV MVTGSWDQTV
   121  KLWDPRTPCN AGTFSQPEKV YTLSVSGDRL IVGTAGRRVL VWDLRNMGYV QQRRESSLKY
   181  QTRCIRAFPN KQGYVLSSIE GRVAVEYLDP SPEVQKKKYA FKCHRLKENN IEQIYPVNAI
   241  SFHNIHNTFA TGGSDGFVNI WDPFNKKRLC QFHRYPTSIA SLAFSNDGTT LAIASSYMYE
   301  MDDTEHPEDG IFIRQVTDAE TKPKSPCT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against BUB3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.22
Highest tissue expression: 97 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 97 nTPM
thymus: 94 nTPM
lymph node: 93 nTPM
tonsil: 82 nTPM
appendix: 60 nTPM
testis: 55 nTPM

Single-cell type

oocytes: 391 nCPM
late primary spermatocytes: 279 nCPM
late spermatids: 232 nCPM
gastric progenitor cells: 200 nCPM
differentiating spermatogonia: 160 nCPM
early primary spermatocytes: 156 nCPM

Immune cell

T-reg: 368 nTPM
total PBMC: 294 nTPM
memory CD8 T-cell: 251 nTPM
naive CD8 T-cell: 250 nTPM
gdT-cell: 247 nTPM
NK-cell: 242 nTPM

Brain region

midbrain: 104 nTPM
white matter: 98 nTPM
cerebral cortex: 95 nTPM
medulla oblongata: 87 nTPM
pons: 85 nTPM
hippocampal formation: 78 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.42
gnomAD pLI: 0.81
gnomAD missense Z: 1.98
DepMap mean gene effect: -2.13
DepMap dependency class: pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

ubiquitin binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of BUB3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads BUB3 as an antibody target. Whether an autoantibody or antibody against BUB3 could matter depends on whether native BUB3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

BUB3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label BUB3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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