BID
BH3-interacting domain death agonist
Also known as: BID_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P55957
- Gene
- BID
- Ensembl
- ENSG00000015475
- Chromosome
- 22
- Canonical length
- 195 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Cytosol
OverviewNCBI Gene
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]
Canonical amino-acid sequenceUniProt
195 residues, UniProt reviewed canonical sequence.
>P55957|BID
1 MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ WEGYDELQTD
61 GNRSSHSRLG RIEADSESQE DIIRNIARHL AQVGDSMDRS IPPGLVNGLA LQLRNTSRSE
121 EDRNRDLATA LEQLLQAYPR DMEKEKTMLV LALLLAKKVA SHTPSLLRDV FHTTVNFINQ
181 NLRTYVRSLA RNGMDLocalizationUniProt · AlphaFold · HPA
Whether an antibody against BID can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Other membrane
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.36
- Highest tissue expression
- 98 nTPM
Expression across tissuesHPA
Tissue
- bone marrow: 98 nTPM
- liver: 45 nTPM
- cerebral cortex: 39 nTPM
- spleen: 39 nTPM
- retina: 34 nTPM
- lymph node: 33 nTPM
Single-cell type
- neutrophils: 1,074 nCPM
- cdc: 455 nCPM
- monocytes: 343 nCPM
- pdcs: 260 nCPM
- neutrophil progenitors: 175 nCPM
- hofbauer cells: 164 nCPM
Immune cell
- non-classical monocyte: 991 nTPM
- neutrophil: 931 nTPM
- intermediate monocyte: 750 nTPM
- eosinophil: 694 nTPM
- total PBMC: 428 nTPM
- classical monocyte: 418 nTPM
Brain region
- thalamus: 30 nTPM
- cerebral cortex: 30 nTPM
- pons: 27 nTPM
- spinal cord: 26 nTPM
- white matter: 26 nTPM
- midbrain: 25 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.92
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.1
- DepMap mean gene effect
- 0.07
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- apoptotic mitochondrial changes
- establishment of protein localization to membrane
- extrinsic apoptotic signaling pathway via death domain receptors
- hepatocyte apoptotic process
- mitochondrial ATP synthesis coupled electron transport
- mitochondrial outer membrane permeabilization
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
- neuron apoptotic process
- positive regulation of apoptotic process
- positive regulation of extrinsic apoptotic signaling pathway
- positive regulation of fibroblast apoptotic process
- positive regulation of intrinsic apoptotic signaling pathway
- positive regulation of mitochondrial membrane potential
- positive regulation of protein-containing complex assembly
- positive regulation of release of cytochrome c from mitochondria
- protein targeting to mitochondrion
- protein-containing complex assembly
- regulation of epithelial cell proliferation
- regulation of G1/S transition of mitotic cell cycle
- regulation of T cell proliferation
- release of cytochrome c from mitochondria
- signal transduction in response to DNA damage
- supramolecular fiber organization
Molecular functions
- cysteine-type endopeptidase regulator activity involved in apoptotic process
- death receptor binding
- ubiquitin protein ligase binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
- Apoptosis regulator, Bcl-2, BH3 motif, conserved site
- Bcl-2-like superfamily
- BH3-interacting domain death agonist
- BH3 interacting domain (BID)
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of BID in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads BID as an antibody target. Whether an autoantibody or antibody against BID could matter depends on whether native BID is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
BID is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label BID as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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