Seroatlas · Human Serome Atlas

ZBTB25

Zinc finger and BTB domain-containing protein 25

Also known as: C14orf51, KUP, ZBT25_HUMAN, ZNF46

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P24278
Gene: ZBTB25
Ensembl: ENSG00000089775
Chromosome: 14
Canonical length: 435 aa
Protein class: Plasma proteins, Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm

OverviewNCBI Gene

Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II; regulation of cytokine production; and regulation of immune system process. Located in nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

435 residues, UniProt reviewed canonical sequence.

>P24278|ZBTB25
     1  MDTASHSLVL LQQLNMQREF GFLCDCTVAI GDVYFKAHRA VLAAFSNYFK MIFIHQTSEC
    61  IKIQPTDIQP DIFSYLLHIM YTGKGPKQIV DHSRLEEGIR FLHADYLSHI ATEMNQVFSP
   121  ETVQSSNLYG IQISTTQKTV VKQGLEVKEA PSSNSGNRAA VQGDHPQLQL SLAIGLDDGT
   181  ADQQRACPAT QALEEHQKPP VSIKQERCDP ESVISQSHPS PSSEVTGPTF TENSVKIHLC
   241  HYCGERFDSR SNLRQHLHTH VSGSLPFGVP ASILESNDLG EVHPLNENSE ALECRRLSSF
   301  IVKENEQQPD HTNRGTTEPL QISQVSLISK DTEPVELNCN FSFSRKRKMS CTICGHKFPR
   361  KSQLLEHMYT HKGKSYRYNR CQRFGNALAQ RFQPYCDSWS DVSLKSSRLS QEHLDLPCAL
   421  ESELTQENVD TILVE

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ZBTB25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.54
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

thymus: 12 nTPM
skeletal muscle: 11 nTPM
tonsil: 10 nTPM
lymph node: 10 nTPM
retina: 10 nTPM
heart muscle: 10 nTPM

Single-cell type

cardiomyocytes: 210 nCPM
adipocytes: 84 nCPM
myonuclei: 82 nCPM
late spermatids: 81 nCPM
early spermatids: 70 nCPM
thyrotrophs: 69 nCPM

Immune cell

naive CD8 T-cell: 11 nTPM
naive CD4 T-cell: 11 nTPM
memory CD4 T-cell: 9.3 nTPM
T-reg: 8.9 nTPM
MAIT T-cell: 8.6 nTPM
total PBMC: 7.5 nTPM

Brain region

white matter: 38 nTPM
medulla oblongata: 35 nTPM
thalamus: 34 nTPM
cerebellum: 33 nTPM
cerebral cortex: 33 nTPM
basal ganglia: 32 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.13
gnomAD pLI: 0
gnomAD missense Z: 0.62
DepMap mean gene effect: -0.04
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

nucleoplasm

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of ZBTB25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads ZBTB25 as an antibody target. Whether an autoantibody or antibody against ZBTB25 could matter depends on whether native ZBTB25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

ZBTB25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label ZBTB25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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