Seroatlas · Human Serome Atlas

USP54

Ubiquitin carboxyl-terminal hydrolase 54

Also known as: bA137L10.3, bA137L10.4, C10orf29, FLJ37318, UBP54_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q70EL1
Gene: USP54
Ensembl: ENSG00000166348
Chromosome: 10
Canonical length: 1684 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Mitochondria

OverviewNCBI Gene

Enables K63-linked deubiquitinase activity and cysteine-type deubiquitinase activity. Predicted to be involved in protein deubiquitination and proteolysis. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1684 residues, UniProt reviewed canonical sequence.

>Q70EL1|USP54
     1  MSWKRNYFSG GRGSVQGMFA PRSSTSIAPS KGLSNEPGQN SCFLNSALQV LWHLDIFRRS
    61  FRQLTTHKCM GDSCIFCALK GIFNQFQCSS EKVLPSDTLR SALAKTFQDE QRFQLGIMDD
   121  AAECFENLLM RIHFHIADET KEDICTAQHC ISHQKFAMTL FEQCVCTSCG ATSDPLPFIQ
   181  MVHYISTTSL CNQAICMLER REKPSPSMFG ELLQNASTMG DLRNCPSNCG ERIRIRRVLM
   241  NAPQIITIGL VWDSDHSDLA EDVIHSLGTC LKLGDLFFRV TDDRAKQSEL YLVGMICYYG
   301  KHYSTFFFQT KIRKWMYFDD AHVKEIGPKW KDVVTKCIKG HYQPLLLLYA DPQGTPVSTQ
   361  DLPPQAEFQS YSRTCYDSED SGREPSISSD TRTDSSTESY PYKHSHHESV VSHFSSDSQG
   421  TVIYNVENDS MSQSSRDTGH LTDSECNQKH TSKKGSLIER KRSSGRVRRK GDEPQASGYH
   481  SEGETLKEKQ APRNASKPSS STNRLRDFKE TVSNMIHNRP SLASQTNVGS HCRGRGGDQP
   541  DKKPPRTLPL HSRDWEIEST SSESKSSSSS KYRPTWRPKR ESLNIDSIFS KDKRKHCGYT
   601  QLSPFSEDSA KEFIPDEPSK PPSYDIKFGG PSPQYKRWGP ARPGSHLLEQ HPRLIQRMES
   661  GYESSERNSS SPVSLDAALP ESSNVYRDPS AKRSAGLVPS WRHIPKSHSS SILEVDSTAS
   721  MGGWTKSQPF SGEEISSKSE LDELQEEVAR RAQEQELRRK REKELEAAKG FNPHPSRFMD
   781  LDELQNQGRS DGFERSLQEA ESVFEESLHL EQKGDCAAAL ALCNEAISKL RLALHGASCS
   841  THSRALVDKK LQISIRKARS LQDRMQQQQS PQQPSQPSAC LPTQAGTLSQ PTSEQPIPLQ
   901  VLLSQEAQLE SGMDTEFGAS SFFHSPASCH ESHSSLSPES SAPQHSSPSR SALKLLTSVE
   961  VDNIEPSAFH RQGLPKAPGW TEKNSHHSWE PLDAPEGKLQ GSRCDNSSCS KLPPQEGRGI
  1021  AQEQLFQEKK DPANPSPVMP GIATSERGDE HSLGCSPSNS SAQPSLPLYR TCHPIMPVAS
  1081  SFVLHCPDPV QKTNQCLQGQ SLKTSLTLKV DRGSEETYRP EFPSTKGLVR SLAEQFQRMQ
  1141  GVSMRDSTGF KDRSLSGSLR KNSSPSDSKP PFSQGQEKGH WPWAKQQSSL EGGDRPLSWE
  1201  ESTEHSSLAL NSGLPNGETS SGGQPRLAEP DIYQEKLSQV RDVRSKDLGS STDLGTSLPL
  1261  DSWVNITRFC DSQLKHGAPR PGMKSSPHDS HTCVTYPERN HILLHPHWNQ DTEQETSELE
  1321  SLYQASLQAS QAGCSGWGQQ DTAWHPLSQT GSADGMGRRL HSAHDPGLSK TSTAEMEHGL
  1381  HEARTVRTSQ ATPCRGLSRE CGEDEQYSAE NLRRISRSLS GTVVSEREEA PVSSHSFDSS
  1441  NVRKPLETGH RCSSSSSLPV IHDPSVFLLG PQLYLPQPQF LSPDVLMPTM AGEPNRLPGT
  1501  SRSVQQFLAM CDRGETSQGA KYTGRTLNYQ SLPHRSRTDN SWAPWSETNQ HIGTRFLTTP
  1561  GCNPQLTYTA TLPERSKGLQ VPHTQSWSDL FHSPSHPPIV HPVYPPSSSL HVPLRSAWNS
  1621  DPVPGSRTPG PRRVDMPPDD DWRQSSYASH SGHRRTVGEG FLFVLSDAPR REQIRARVLQ
  1681  HSQW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against USP54 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 123 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 123 nTPM
spinal cord: 68 nTPM
midbrain: 46 nTPM
thyroid gland: 39 nTPM
hippocampal formation: 37 nTPM
basal ganglia: 33 nTPM

Single-cell type

myonuclei: 1,715 nCPM
oligodendrocytes: 698 nCPM
alveolar cells type 2: 626 nCPM
transitional alveolar cells: 539 nCPM
thymic myoid cells: 458 nCPM
oligodendrocyte progenitor cells: 371 nCPM

Immune cell

MAIT T-cell: 0.7 nTPM
naive CD4 T-cell: 0.7 nTPM
gdT-cell: 0.6 nTPM
T-reg: 0.6 nTPM
memory CD4 T-cell: 0.5 nTPM
non-classical monocyte: 0.5 nTPM

Brain region

white matter: 229 nTPM
basal ganglia: 169 nTPM
medulla oblongata: 167 nTPM
midbrain: 160 nTPM
pons: 148 nTPM
cerebellum: 139 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.55
gnomAD pLI: 0
gnomAD missense Z: 2.14
DepMap mean gene effect: -0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of USP54 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads USP54 as an antibody target. Whether an autoantibody or antibody against USP54 could matter depends on whether native USP54 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

USP54 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label USP54 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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