UGT1A4

Protein identityUniProt · HPA

UniProt accession

P22310

Gene

UGT1A4

Ensembl

ENSG00000244474

Chromosome

2

Canonical length

534 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Predicted membrane proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. This enzyme has some glucuronidase activity towards bilirubin, although is is more active on amines, steroids, and sapogenins. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

534 residues, UniProt reviewed canonical sequence.

>P22310|UGT1A4
     1  MARGLQVPLP RLATGLLLLL SVQPWAESGK VLVVPTDGSP WLSMREALRE LHARGHQAVV
    61  LTPEVNMHIK EEKFFTLTAY AVPWTQKEFD RVTLGYTQGF FETEHLLKRY SRSMAIMNNV
   121  SLALHRCCVE LLHNEALIRH LNATSFDVVL TDPVNLCGAV LAKYLSIPAV FFWRYIPCDL
   181  DFKGTQCPNP SSYIPKLLTT NSDHMTFLQR VKNMLYPLAL SYICHTFSAP YASLASELFQ
   241  REVSVVDLVS YASVWLFRGD FVMDYPRPIM PNMVFIGGIN CANGKPLSQE FEAYINASGE
   301  HGIVVFSLGS MVSEIPEKKA MAIADALGKI PQTVLWRYTG TRPSNLANNT ILVKWLPQND
   361  LLGHPMTRAF ITHAGSHGVY ESICNGVPMV MMPLFGDQMD NAKRMETKGA GVTLNVLEMT
   421  SEDLENALKA VINDKSYKEN IMRLSSLHKD RPVEPLDLAV FWVEFVMRHK GAPHLRPAAH
   481  DLTWYQYHSL DVIGFLLAVV LTVAFITFKC CAYGYRKCLG KKGRVKKAHK SKTH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UGT1A4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.28

Highest tissue expression

368 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.13

gnomAD pLI

0

gnomAD missense Z

-1.65

DepMap mean gene effect

-0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• bilirubin conjugation
• glucuronate metabolic process
• heme catabolic process
• negative regulation of fatty acid metabolic process
• vitamin D3 metabolic process

Molecular functions

• enzyme binding
• enzyme inhibitor activity
• glucuronosyltransferase activity
• protein heterodimerization activity
• protein homodimerization activity

Cellular components

• endoplasmic reticulum
• endoplasmic reticulum membrane

Protein domainsUniProt · Pfam · InterPro

• UDP-glucuronosyl/UDP-glucosyltransferase
• UDP-glycosyltransferase family, conserved site
• UDP-glycosyltransferase
• UDP-glucoronosyl and UDP-glucosyl transferase

KeywordsUniProt

• Endoplasmic reticulum
• Glycoprotein
• Glycosyltransferase
• Lipid metabolism
• Membrane
• Signal
• Transferase
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of UGT1A4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• UGT1A1
• UGT1A10
• UGT1A3
• UGT1A6
• UGT1A7
• UGT1A8
• UGT1A9
• UGT1A5