UBA3

Protein identityUniProt · HPA

UniProt accession

Q8TBC4

Gene

UBA3

Ensembl

ENSG00000144744

Chromosome

3

Canonical length

463 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Plasma membrane,Centrosome,Cytosol

OverviewNCBI Gene

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

463 residues, UniProt reviewed canonical sequence.

>Q8TBC4|UBA3
     1  MADGEEPEKK RRRIEELLAE KMAVDGGCGD TGDWEGRWNH VKKFLERSGP FTHPDFEPST
    61  ESLQFLLDTC KVLVIGAGGL GCELLKNLAL SGFRQIHVID MDTIDVSNLN RQFLFRPKDI
   121  GRPKAEVAAE FLNDRVPNCN VVPHFNKIQD FNDTFYRQFH IIVCGLDSII ARRWINGMLI
   181  SLLNYEDGVL DPSSIVPLID GGTEGFKGNA RVILPGMTAC IECTLELYPP QVNFPMCTIA
   241  SMPRLPEHCI EYVRMLQWPK EQPFGEGVPL DGDDPEHIQW IFQKSLERAS QYNIRGVTYR
   301  LTQGVVKRII PAVASTNAVI AAVCATEVFK IATSAYIPLN NYLVFNDVDG LYTYTFEAER
   361  KENCPACSQL PQNIQFSPSA KLQEVLDYLT NSASLQMKSP AITATLEGKN RTLYLQSVTS
   421  IEERTRPNLS KTLKELGLVD GQELAVADVT TPQTVLFKLH FTS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UBA3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.29

Highest tissue expression

62 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.41

gnomAD pLI

0.5

gnomAD missense Z

1.17

DepMap mean gene effect

-0.61

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• endomitotic cell cycle
• post-translational protein modification
• protein modification process
• protein neddylation
• proteolysis
• regulation of cell cycle

Molecular functions

• ATP binding
• identical protein binding
• NEDD8 activating enzyme activity
• NEDD8 transferase activity
• protein heterodimerization activity

Cellular components

• cytoplasm
• cytosol
• nucleus
• protein-containing complex

Protein domainsUniProt · Pfam · InterPro

• THIF-type NAD/FAD binding fold
• Ubiquitin activating enzyme, alpha domain superfamily
• Ubiquitin-activating enzyme E1, Cys active site
• Ubiquitin-activating enzyme-like
• ThiF/MoeB/HesA family
• ThiF family
• E2 binding
• NEDD8-activating enzyme E1 catalytic subunit, N-terminal domain
• E2 binding domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Cell cycle
• Ligase
• Nucleotide-binding
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of UBA3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NEDD8
• TBATA
• UBE2F
• UBE2M
• NAE1