Seroatlas · Human Serome Atlas

UBA1

Ubiquitin-like modifier-activating enzyme 1

Also known as: A1S9T, CFAP124, GXP1, POC20, UBA1_HUMAN, UBE1, UBE1X

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
P22314
Gene
UBA1
Ensembl
ENSG00000130985
Chromosome
X
Canonical length
1058 aa
Protein class
Cancer-related genes, Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Plasma proteins, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1058 residues, UniProt reviewed canonical sequence.

>P22314|UBA1
     1  MSSSPLSKKR RVSGPDPKPG SNCSPAQSVL SEVPSVPTNG MAKNGSEADI DEGLYSRQLY
    61  VLGHEAMKRL QTSSVLVSGL RGLGVEIAKN IILGGVKAVT LHDQGTAQWA DLSSQFYLRE
   121  EDIGKNRAEV SQPRLAELNS YVPVTAYTGP LVEDFLSGFQ VVVLTNTPLE DQLRVGEFCH
   181  NRGIKLVVAD TRGLFGQLFC DFGEEMILTD SNGEQPLSAM VSMVTKDNPG VVTCLDEARH
   241  GFESGDFVSF SEVQGMVELN GNQPMEIKVL GPYTFSICDT SNFSDYIRGG IVSQVKVPKK
   301  ISFKSLVASL AEPDFVVTDF AKFSRPAQLH IGFQALHQFC AQHGRPPRPR NEEDAAELVA
   361  LAQAVNARAL PAVQQNNLDE DLIRKLAYVA AGDLAPINAF IGGLAAQEVM KACSGKFMPI
   421  MQWLYFDALE CLPEDKEVLT EDKCLQRQNR YDGQVAVFGS DLQEKLGKQK YFLVGAGAIG
   481  CELLKNFAMI GLGCGEGGEI IVTDMDTIEK SNLNRQFLFR PWDVTKLKSD TAAAAVRQMN
   541  PHIRVTSHQN RVGPDTERIY DDDFFQNLDG VANALDNVDA RMYMDRRCVY YRKPLLESGT
   601  LGTKGNVQVV IPFLTESYSS SQDPPEKSIP ICTLKNFPNA IEHTLQWARD EFEGLFKQPA
   661  ENVNQYLTDP KFVERTLRLA GTQPLEVLEA VQRSLVLQRP QTWADCVTWA CHHWHTQYSN
   721  NIRQLLHNFP PDQLTSSGAP FWSGPKRCPH PLTFDVNNPL HLDYVMAAAN LFAQTYGLTG
   781  SQDRAAVATF LQSVQVPEFT PKSGVKIHVS DQELQSANAS VDDSRLEELK ATLPSPDKLP
   841  GFKMYPIDFE KDDDSNFHMD FIVAASNLRA ENYDIPSADR HKSKLIAGKI IPAIATTTAA
   901  VVGLVCLELY KVVQGHRQLD SYKNGFLNLA LPFFGFSEPL AAPRHQYYNQ EWTLWDRFEV
   961  QGLQPNGEEM TLKQFLDYFK TEHKLEITML SQGVSMLYSF FMPAAKLKER LDQPMTEIVS
  1021  RVSKRKLGRH VRALVLELCC NDESGEDVEV PYVRYTIR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against UBA1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
190 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 190 nTPM
  • choroid plexus: 169 nTPM
  • thyroid gland: 152 nTPM
  • adrenal gland: 150 nTPM
  • esophagus: 149 nTPM
  • kidney: 139 nTPM

Single-cell type

  • esophageal apical cells: 225 nCPM
  • esophageal suprabasal cells: 157 nCPM
  • colonocytes: 124 nCPM
  • neutrophils: 122 nCPM
  • adrenal cortex cells: 120 nCPM
  • alveolar cells type 1: 118 nCPM

Immune cell

  • intermediate monocyte: 51 nTPM
  • non-classical monocyte: 50 nTPM
  • neutrophil: 45 nTPM
  • myeloid DC: 43 nTPM
  • eosinophil: 41 nTPM
  • T-reg: 40 nTPM

Brain region

  • choroid plexus: 236 nTPM
  • white matter: 198 nTPM
  • cerebral cortex: 192 nTPM
  • thalamus: 187 nTPM
  • pons: 182 nTPM
  • medulla oblongata: 179 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about UBA1.

Disease | AllUniProt

Conditions UBA1 is implicated in, by any mechanism.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.11
gnomAD pLI
1
gnomAD missense Z
3.48
DepMap mean gene effect
-1.75
DepMap dependency class
pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of UBA1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads UBA1 as an antibody target. Whether an autoantibody or antibody against UBA1 could matter depends on whether native UBA1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

UBA1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label UBA1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/UBA1. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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