Seroatlas · Human Serome Atlas

TNFSF14

Tumor necrosis factor ligand superfamily member 14

Also known as: CD258, HVEM-L, LIGHT, LTg, TNF14_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O43557
Gene
TNFSF14
Ensembl
ENSG00000125735
Chromosome
19
Canonical length
240 aa
Protein class
CD markers, Predicted intracellular proteins, Predicted membrane proteins, Predicted secreted proteins
Subcellular location
Vesicles
Secretome location
Secreted to blood
Quaternary structure
Homotrimer

OverviewNCBI Gene

The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF14, which is a member of the tumor necrosis factor receptor superfamily, and which is also known as a herpesvirus entry mediator (HVEM). This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

240 residues, UniProt reviewed canonical sequence.

>O43557|TNFSF14
     1  MEESVVRPSV FVVDGQTDIP FTRLGRSHRR QSCSVARVGL GLLLLLMGAG LAVQGWFLLQ
    61  LHWRLGEMVT RLPDGPAGSW EQLIQERRSH EVNPAAHLTG ANSSLTGSGG PLLWETQLGL
   121  AFLRGLSYHD GALVVTKAGY YYIYSKVQLG GVGCPLGLAS TITHGLYKRT PRYPEELELL
   181  VSQQSPCGRA TSSSRVWWDS SFLGGVVHLE AGEKVVVRVL DERLVRLRDG TRSYFGAFMV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TNFSF14 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.4
Highest tissue expression
93 nTPM

Expression across tissuesHPA

Tissue

  • liver: 93 nTPM
  • adipose tissue: 24 nTPM
  • fallopian tube: 6.2 nTPM
  • small intestine: 5.1 nTPM
  • ovary: 4.4 nTPM
  • lung: 4.2 nTPM

Single-cell type

  • neutrophils: 156 nCPM
  • mesothelial cells: 68 nCPM
  • epicardial cells: 55 nCPM
  • nk-cells: 53 nCPM
  • innate lymphoid cells: 41 nCPM
  • t-cells: 36 nCPM

Immune cell

  • neutrophil: 200 nTPM
  • basophil: 141 nTPM
  • eosinophil: 115 nTPM
  • MAIT T-cell: 61 nTPM
  • gdT-cell: 42 nTPM
  • memory CD8 T-cell: 34 nTPM

Brain region

  • cerebral cortex: 13 nTPM
  • thalamus: 6.1 nTPM
  • amygdala: 4.3 nTPM
  • pons: 3.3 nTPM
  • white matter: 3.2 nTPM
  • choroid plexus: 2.6 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about TNFSF14.

Disease | ImmuneIEDB

Conditions an epitope on TNFSF14 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.8
gnomAD pLI
0.05
gnomAD missense Z
0.64
DepMap mean gene effect
-0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TNFSF14 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TNFSF14 as an antibody target. Whether an autoantibody or antibody against TNFSF14 could matter depends on whether native TNFSF14 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TNFSF14 is annotated at the cell surface, where native TNFSF14 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TNFSF14 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/TNFSF14. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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