Seroatlas · Human Serome Atlas

TMED5

Transmembrane emp24 domain-containing protein 5

Also known as: CGI-100, p24g2, p24gamma2, TMED5_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y3A6
Gene: TMED5
Ensembl: ENSG00000117500
Chromosome: 1
Canonical length: 229 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

Involved in Golgi ribbon formation. Located in cis-Golgi network; endoplasmic reticulum exit site; and endoplasmic reticulum-Golgi intermediate compartment. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

229 residues, UniProt reviewed canonical sequence.

>Q9Y3A6|TMED5
     1  MGDKIWLPFP VLLLAALPPV LLPGAAGFTP SLDSDFTFTL PAGQKECFYQ PMPLKASLEI
    61  EYQVLDGAGL DIDFHLASPE GKTLVFEQRK SDGVHTVETE VGDYMFCFDN TFSTISEKVI
   121  FFELILDNMG EQAQEQEDWK KYITGTDILD MKLEDILESI NSIKSRLSKS GHIQTLLRAF
   181  EARDRNIQES NFDRVNFWSM VNLVVMVVVS AIQVYMLKSL FEDKRKSRT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMED5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 49 nTPM

Expression across tissuesHPA

Tissue

liver: 49 nTPM
bone marrow: 29 nTPM
kidney: 26 nTPM
skeletal muscle: 19 nTPM
adipose tissue: 18 nTPM
appendix: 18 nTPM

Single-cell type

neutrophils: 261 nCPM
late spermatids: 232 nCPM
plasma cells: 187 nCPM
monocytes: 186 nCPM
neutrophil progenitors: 162 nCPM
monocyte progenitors: 157 nCPM

Immune cell

basophil: 57 nTPM
eosinophil: 46 nTPM
non-classical monocyte: 36 nTPM
intermediate monocyte: 33 nTPM
classical monocyte: 33 nTPM
total PBMC: 29 nTPM

Brain region

choroid plexus: 36 nTPM
white matter: 23 nTPM
thalamus: 22 nTPM
hypothalamus: 21 nTPM
medulla oblongata: 20 nTPM
spinal cord: 19 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.74
gnomAD pLI: 0
gnomAD missense Z: 0.67
DepMap mean gene effect: -0.11
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMED5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMED5 as an antibody target. Whether an autoantibody or antibody against TMED5 could matter depends on whether native TMED5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMED5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMED5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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