Seroatlas · Human Serome Atlas

TMED3

Transmembrane emp24 domain-containing protein 3

Also known as: C15orf22, p24B, p24g4, p24gamma4, TMED3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y3Q3
Gene: TMED3
Ensembl: ENSG00000166557
Chromosome: 15
Canonical length: 217 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins, Transporters
Subcellular location: Golgi apparatus

OverviewNCBI Gene

Predicted to be involved in Golgi organization; endoplasmic reticulum to Golgi vesicle-mediated transport; and intracellular protein transport. Located in Golgi apparatus; endoplasmic reticulum; and endoplasmic reticulum-Golgi intermediate compartment. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

217 residues, UniProt reviewed canonical sequence.

>Q9Y3Q3|TMED3
     1  MGSTVPRSAS VLLLLLLLRR AEQPCGAELT FELPDNAKQC FHEEVEQGVK FSLDYQVITG
    61  GHYDVDCYVE DPQGNTIYRE TKKQYDSFTY RAEVKGVYQF CFSNEFSTFS HKTVYFDFQV
   121  GDEPPILPDM GNRVTALTQM ESACVTIHEA LKTVIDSQTH YRLREAQDRA RAEDLNSRVS
   181  YWSVGETIAL FVVSFSQVLL LKSFFTEKRP ISRAVHS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TMED3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.41
Highest tissue expression: 386 nTPM

Expression across tissuesHPA

Tissue

salivary gland: 386 nTPM
pancreas: 233 nTPM
esophagus: 139 nTPM
epididymis: 131 nTPM
skin: 112 nTPM
pituitary gland: 91 nTPM

Single-cell type

esophageal apical cells: 1,058 nCPM
salivary acinar cells: 684 nCPM
esophageal suprabasal cells: 468 nCPM
lacrimal acinar cells: 448 nCPM
extravillous trophoblasts: 414 nCPM
epididymal principal cells: 382 nCPM

Immune cell

plasmacytoid DC: 109 nTPM
myeloid DC: 107 nTPM
non-classical monocyte: 96 nTPM
eosinophil: 95 nTPM
total PBMC: 92 nTPM
intermediate monocyte: 85 nTPM

Brain region

hypothalamus: 50 nTPM
choroid plexus: 47 nTPM
pons: 39 nTPM
white matter: 36 nTPM
medulla oblongata: 30 nTPM
midbrain: 29 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.99
gnomAD pLI: 0.02
gnomAD missense Z: 0.3
DepMap mean gene effect: 0.04
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TMED3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TMED3 as an antibody target. Whether an autoantibody or antibody against TMED3 could matter depends on whether native TMED3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TMED3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label TMED3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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