Seroatlas · Human Serome Atlas

TJP3

Tight junction protein ZO-3

Also known as: ZO-3, ZO3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O95049
Gene: TJP3
Ensembl: ENSG00000105289
Chromosome: 19
Canonical length: 919 aa
Protein class: Predicted intracellular proteins, Transporters
Subcellular location: Nucleoplasm,Plasma membrane,Cell Junctions

OverviewNCBI Gene

The protein encoded by this gene is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK)-domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by this gene plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. This gene has a partial pseudogene on chromosome 1. [provided by RefSeq, May 2012]

Canonical amino-acid sequenceUniProt

919 residues, UniProt reviewed canonical sequence.

>O95049|TJP3
     1  MEELTIWEQH TATLSKDPRR GFGIAISGGR DRPGGSMVVS DVVPGGPAEG RLQTGDHIVM
    61  VNGVSMENAT SAFAIQILKT CTKMANITVK RPRRIHLPAT KASPSSPGRQ DSDEDDGPQR
   121  VEEVDQGRGY DGDSSSGSGR SWDERSRRPR PGRRGRAGSH GRRSPGGGSE ANGLALVSGF
   181  KRLPRQDVQM KPVKSVLVKR RDSEEFGVKL GSQIFIKHIT DSGLAARHRG LQEGDLILQI
   241  NGVSSQNLSL NDTRRLIEKS EGKLSLLVLR DRGQFLVNIP PAVSDSDSSP LEDISDLASE
   301  LSQAPPSHIP PPPRHAQRSP EASQTDSPVE SPRLRRESSV DSRTISEPDE QRSELPRESS
   361  YDIYRVPSSQ SMEDRGYSPD TRVVRFLKGK SIGLRLAGGN DVGIFVSGVQ AGSPADGQGI
   421  QEGDQILQVN DVPFQNLTRE EAVQFLLGLP PGEEMELVTQ RKQDIFWKMV QSRVGDSFYI
   481  RTHFELEPSP PSGLGFTRGD VFHVLDTLHP GPGQSHARGG HWLAVRMGRD LREQERGIIP
   541  NQSRAEQLAS LEAAQRAVGV GPGSSAGSNA RAEFWRLRGL RRGAKKTTQR SREDLSALTR
   601  QGRYPPYERV VLREASFKRP VVILGPVADI AMQKLTAEMP DQFEIAETVS RTDSPSKIIK
   661  LDTVRVIAEK DKHALLDVTP SAIERLNYVQ YYPIVVFFIP ESRPALKALR QWLAPASRRS
   721  TRRLYAQAQK LRKHSSHLFT ATIPLNGTSD TWYQELKAII REQQTRPIWT AEDQLDGSLE
   781  DNLDLPHHGL ADSSADLSCD SRVNSDYETD GEGGAYTDGE GYTDGEGGPY TDVDDEPPAP
   841  ALARSSEPVQ ADESQSPRDR GRISAHQGAQ VDSRHPQGQW RQDSMRTYER EALKKKFMRV
   901  HDAESSDEDG YDWGPATDL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against TJP3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.45
Highest tissue expression: 41 nTPM

Expression across tissuesHPA

Tissue

duodenum: 41 nTPM
small intestine: 35 nTPM
colon: 33 nTPM
stomach: 26 nTPM
esophagus: 25 nTPM
salivary gland: 21 nTPM

Single-cell type

early spermatids: 404 nCPM
late primary spermatocytes: 322 nCPM
esophageal apical cells: 313 nCPM
colonocytes: 257 nCPM
enterocytes: 198 nCPM
respiratory ciliated cells: 191 nCPM

Immune cell

MAIT T-cell: 3.9 nTPM
T-reg: 3.8 nTPM
naive CD4 T-cell: 3.3 nTPM
memory CD4 T-cell: 3.1 nTPM
memory CD8 T-cell: 2.4 nTPM
gdT-cell: 1.2 nTPM

Brain region

choroid plexus: 16 nTPM
midbrain: 1.8 nTPM
hippocampal formation: 1.7 nTPM
cerebral cortex: 1.5 nTPM
white matter: 1.4 nTPM
pons: 1.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.08
gnomAD pLI: 0
gnomAD missense Z: -0.67
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

cell adhesion molecule binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of TJP3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads TJP3 as an antibody target. Whether an autoantibody or antibody against TJP3 could matter depends on whether native TJP3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

TJP3 is annotated at the cell surface, where native TJP3 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label TJP3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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