Seroatlas · Human Serome Atlas

SPRY3

Protein sprouty homolog 3

Also known as: HSPRY3, SPY3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O43610
Gene
SPRY3
Ensembl
ENSG00000168939
Chromosome
X
Canonical length
288 aa
Protein class
Predicted membrane proteins
Subcellular location
Nucleoli,Cytosol

OverviewNCBI Gene

Involved in negative regulation of MAPK cascade. Predicted to be located in cytoplasm and membrane. Predicted to be active in cytosol. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

288 residues, UniProt reviewed canonical sequence.

>O43610|SPRY3
     1  MDAAVTDDFQ QILPIEQLRS THASNDYVER PPAPCKQALS SPSLIVQTHK SDWSLATMPT
    61  SLPRSLSQCH QLQPLPQHLS QSSIASSMSH STTASDQRLL ASITPSPSGQ SIIRTQPGAG
   121  VHPKADGALK GEAEQSAGHP SEHLFICEEC GRCKCVPCTA ARPLPSCWLC NQRCLCSAES
   181  LLDYGTCLCC VKGLFYHCST DDEDNCADEP CSCGPSSCFV RWAAMSLISL FLPCLCCYLP
   241  TRGCLHLCQQ GYDSLRRPGC RCKRHTNTVC RKISSGSAPF PKAQEKSV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPRY3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.56
Highest tissue expression
4.2 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 4.2 nTPM
  • epididymis: 2.5 nTPM
  • bone marrow: 1.5 nTPM
  • ovary: 1.5 nTPM
  • adrenal gland: 1.3 nTPM
  • retina: 1.3 nTPM

Single-cell type

  • cardiomyocytes: 743 nCPM
  • myonuclei: 301 nCPM
  • neutrophil progenitors: 203 nCPM
  • endometrial ciliated cells: 163 nCPM
  • neutrophils: 154 nCPM
  • endometrial luminal cells: 126 nCPM

Immune cell

  • basophil: 0.1 nTPM
  • neutrophil: 0.1 nTPM
  • NK-cell: 0.1 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM

Brain region

  • cerebellum: 17 nTPM
  • pons: 10 nTPM
  • medulla oblongata: 9.8 nTPM
  • cerebral cortex: 9.1 nTPM
  • thalamus: 8.8 nTPM
  • hippocampal formation: 8.6 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.15
gnomAD pLI
0
gnomAD missense Z
0.02
DepMap mean gene effect
0.1
DepMap dependency class
none

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPRY3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPRY3 as an antibody target. Whether an autoantibody or antibody against SPRY3 could matter depends on whether native SPRY3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPRY3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPRY3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SPRY3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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