Seroatlas · Human Serome Atlas

SPOPL

Speckle-type POZ protein-like

Also known as: BTBD33, SPOPL_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q6IQ16
Gene: SPOPL
Ensembl: ENSG00000144228
Chromosome: 2
Canonical length: 392 aa
Protein class: Predicted intracellular proteins
Subcellular location: Vesicles
Quaternary structure: Homodimer

OverviewNCBI Gene

Enables identical protein binding activity. Involved in negative regulation of protein ubiquitination and proteasome-mediated ubiquitin-dependent protein catabolic process. Part of Cul3-RING ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

392 residues, UniProt reviewed canonical sequence.

>Q6IQ16|SPOPL
     1  MSREPTPPLP GDMSTGPIAE SWCYTQVKVV KFSYMWTINN FSFCREEMGE VLKSSTFSSG
    61  PSDKMKWCLR VNPKGLDDES KDYLSLYLLL VSCPKSEVRA KFKFSLLNAK REETKAMESQ
   121  RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGHTNTNTLK
   181  VPECRLAEDL GNLWENTRFT DCSFFVRGQE FKAHKSVLAA RSPVFNAMFE HEMEESKKNR
   241  VEINDLDPEV FKEMMRFIYT GRAPNLDKMA DNLLAAADKY ALERLKVMCE EALCSNLSVE
   301  NVADTLVLAD LHSAEQLKAQ AIDFINRCSV LRQLGCKDGK NWNSNQATDI METSGWKSMI
   361  QSHPHLVAEA FRALASAQCP QFGIPRKRLK QS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPOPL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.34
Highest tissue expression: 16 nTPM

Expression across tissuesHPA

Tissue

blood vessel: 16 nTPM
bone marrow: 14 nTPM
breast: 12 nTPM
salivary gland: 11 nTPM
lymph node: 11 nTPM
spleen: 11 nTPM

Single-cell type

neutrophils: 610 nCPM
neutrophil progenitors: 298 nCPM
microglia: 140 nCPM
breast hormone-responsive cells: 133 nCPM
monocyte progenitors: 115 nCPM
renal collecting duct intercalated cells: 114 nCPM

Immune cell

neutrophil: 5.7 nTPM
eosinophil: 2.8 nTPM
myeloid DC: 2.2 nTPM
classical monocyte: 1.6 nTPM
intermediate monocyte: 1.5 nTPM
non-classical monocyte: 1.3 nTPM

Brain region

midbrain: 19 nTPM
white matter: 18 nTPM
basal ganglia: 16 nTPM
pons: 16 nTPM
spinal cord: 15 nTPM
medulla oblongata: 15 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.58
gnomAD pLI: 0.01
gnomAD missense Z: 0.96
DepMap mean gene effect: -0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SPOPL in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SPOPL as an antibody target. Whether an autoantibody or antibody against SPOPL could matter depends on whether native SPOPL is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SPOPL is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label SPOPL as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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