SPDEF

Protein identityUniProt · HPA

UniProt accession

O95238

Gene

SPDEF

Ensembl

ENSG00000124664

Chromosome

6

Canonical length

335 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Nucleoli,Cytosol

OverviewNCBI Gene

The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Canonical amino-acid sequenceUniProt

335 residues, UniProt reviewed canonical sequence.

>O95238|SPDEF
     1  MGSASPGLSS VSPSHLLLPP DTVSRTGLEK AAAGAVGLER RDWSPSPPAT PEQGLSAFYL
    61  SYFDMLYPED SSWAAKAPGA SSREEPPEEP EQCPVIDSQA PAGSLDLVPG GLTLEEHSLE
   121  QVQSMVVGEV LKDIETACKL LNITADPMDW SPSNVQKWLL WTEHQYRLPP MGKAFQELAG
   181  KELCAMSEEQ FRQRSPLGGD VLHAHLDIWK SAAWMKERTS PGAIHYCAST SEESWTDSEV
   241  DSSCSGQPIH LWQFLKELLL KPHSYGRFIR WLNKEKGIFK IEDSAQVARL WGIRKNRPAM
   301  NYDKLSRSIR QYYKKGIIRK PDISQRLVYQ FVHPI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SPDEF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.48

Highest tissue expression

135 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.31

gnomAD pLI

0.97

gnomAD missense Z

0.8

DepMap mean gene effect

0.01

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell differentiation
• epithelial cell fate commitment
• glandular epithelial cell development
• intestinal epithelial cell development
• lung goblet cell differentiation
• negative regulation of cell fate commitment
• negative regulation of transcription by RNA polymerase II
• positive regulation of apoptotic process
• positive regulation of cell fate commitment
• positive regulation of transcription by RNA polymerase II
• regulation of transcription by RNA polymerase II
• transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity, RNA polymerase II-specific
• sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Ets domain
• Pointed domain
• Sterile alpha motif/pointed domain superfamily
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• ETS family
• Ets-domain
• Sterile alpha motif (SAM)/Pointed domain

KeywordsUniProt

• Activator
• DNA-binding
• Nucleus
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of SPDEF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• NKX3-1
• AR