SLIT3

Protein identityUniProt · HPA

UniProt accession

O75094

Gene

SLIT3

Ensembl

ENSG00000184347

Chromosome

5

Canonical length

1523 aa

Protein class

Predicted intracellular proteins, Predicted secreted proteins

Subcellular location

Cell Junctions

Secretome location

Secreted in other tissues

OverviewNCBI Gene

The protein encoded by this gene is secreted, likely interacting with roundabout homolog receptors to effect cell migration. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Canonical amino-acid sequenceUniProt

1523 residues, UniProt reviewed canonical sequence.

>O75094|SLIT3
     1  MAPGWAGVGA AVRARLALAL ALASVLSGPP AVACPTKCTC SAASVDCHGL GLRAVPRGIP
    61  RNAERLDLDR NNITRITKMD FAGLKNLRVL HLEDNQVSVI ERGAFQDLKQ LERLRLNKNK
   121  LQVLPELLFQ STPKLTRLDL SENQIQGIPR KAFRGITDVK NLQLDNNHIS CIEDGAFRAL
   181  RDLEILTLNN NNISRILVTS FNHMPKIRTL RLHSNHLYCD CHLAWLSDWL RQRRTVGQFT
   241  LCMAPVHLRG FNVADVQKKE YVCPAPHSEP PSCNANSISC PSPCTCSNNI VDCRGKGLME
   301  IPANLPEGIV EIRLEQNSIK AIPAGAFTQY KKLKRIDISK NQISDIAPDA FQGLKSLTSL
   361  VLYGNKITEI VKGLFDGLVS LQLLLLNANK INCLRVNTFQ DLQNLNLLSL YDNKLQTISK
   421  GLFAPLQSIQ TLHLAQNPFV CDCHLKWLAD YLQDNPIETS GARCSSPRRL ANKRISQIKS
   481  KKFRCSGSED YRSRFSSECF MDLVCPEKCR CEGTIVDCSN QKLVRIPSHL PEYVTDLRLN
   541  DNEVSVLEAT GIFKKLPNLR KINLSNNKIK EVREGAFDGA ASVQELMLTG NQLETVHGRV
   601  FRGLSGLKTL MLRSNLIGCV SNDTFAGLSS VRLLSLYDNR ITTITPGAFT TLVSLSTINL
   661  LSNPFNCNCH LAWLGKWLRK RRIVSGNPRC QKPFFLKEIP IQDVAIQDFT CDGNEESSCQ
   721  LSPRCPEQCT CMETVVRCSN KGLRALPRGM PKDVTELYLE GNHLTAVPRE LSALRHLTLI
   781  DLSNNSISML TNYTFSNMSH LSTLILSYNR LRCIPVHAFN GLRSLRVLTL HGNDISSVPE
   841  GSFNDLTSLS HLALGTNPLH CDCSLRWLSE WVKAGYKEPG IARCSSPEPM ADRLLLTTPT
   901  HRFQCKGPVD INIVAKCNAC LSSPCKNNGT CTQDPVELYR CACPYSYKGK DCTVPINTCI
   961  QNPCQHGGTC HLSDSHKDGF SCSCPLGFEG QRCEINPDDC EDNDCENNAT CVDGINNYVC
  1021  ICPPNYTGEL CDEVIDHCVP ELNLCQHEAK CIPLDKGFSC ECVPGYSGKL CETDNDDCVA
  1081  HKCRHGAQCV DTINGYTCTC PQGFSGPFCE HPPPMVLLQT SPCDQYECQN GAQCIVVQQE
  1141  PTCRCPPGFA GPRCEKLITV NFVGKDSYVE LASAKVRPQA NISLQVATDK DNGILLYKGD
  1201  NDPLALELYQ GHVRLVYDSL SSPPTTVYSV ETVNDGQFHS VELVTLNQTL NLVVDKGTPK
  1261  SLGKLQKQPA VGINSPLYLG GIPTSTGLSA LRQGTDRPLG GFHGCIHEVR INNELQDFKA
  1321  LPPQSLGVSP GCKSCTVCKH GLCRSVEKDS VVCECRPGWT GPLCDQEARD PCLGHRCHHG
  1381  KCVATGTSYM CKCAEGYGGD LCDNKNDSAN ACSAFKCHHG QCHISDQGEP YCLCQPGFSG
  1441  EHCQQENPCL GQVVREVIRR QKGYASCATA SKVPIMECRG GCGPQCCQPT RSKRRKYVFQ
  1501  CTDGSSFVEE VERHLECGCL ACS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SLIT3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

64 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SLIT3.

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 325 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Familial congenital diaphragmatic hernia

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.32

gnomAD pLI

0.75

gnomAD missense Z

1.94

DepMap mean gene effect

0.07

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• aortic valve morphogenesis
• apoptotic process involved in luteolysis
• atrioventricular valve morphogenesis
• axon extension involved in axon guidance
• axon guidance
• cellular response to hormone stimulus
• negative chemotaxis
• negative regulation of cell growth
• negative regulation of cell population proliferation
• negative regulation of chemokine-mediated signaling pathway
• negative regulation of gene expression
• response to cortisol
• Roundabout signaling pathway
• ventricular septum morphogenesis

Molecular functions

• calcium ion binding
• heparin binding
• Roundabout binding

Cellular components

• extracellular space
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• EGF-type aspartate/asparagine hydroxylation site
• Leucine-rich repeat N-terminal domain
• Cysteine-rich flanking region, C-terminal
• EGF-like domain
• Leucine-rich repeat
• Laminin G domain
• EGF-like calcium-binding domain
• Leucine-rich repeat, typical subtype
• Cystine knot, C-terminal
• Growth factor receptor cysteine-rich domain superfamily
• EGF-like, conserved site
• Concanavalin A-like lectin/glucanase domain superfamily
• EGF-like calcium-binding, conserved site
• Leucine-rich repeat domain superfamily
• Notch and Slit guidance protein
• EGF-like domain
• Leucine rich repeat N-terminal domain
• Leucine rich repeat C-terminal domain
• Laminin G domain
• Human growth factor-like EGF
• Leucine rich repeat

KeywordsUniProt

• Developmental protein
• Differentiation
• Disulfide bond
• EGF-like domain
• Glycoprotein
• Leucine-rich repeat
• Neurogenesis
• Repeat
• Secreted
• Signal