Seroatlas · Human Serome Atlas

SEMA4G

Semaphorin-4G

Also known as: FLJ20590, KIAA1619, SEM4G_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9NTN9
Gene
SEMA4G
Ensembl
ENSG00000095539
Chromosome
10
Canonical length
838 aa
Protein class
Plasma proteins, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Vesicles,Lipid droplets
Secretome location
Intracellular and membrane

OverviewNCBI Gene

Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

838 residues, UniProt reviewed canonical sequence.

>Q9NTN9|SEMA4G
     1  MWGRLWPLLL SILTATAVPG PSLRRPSREL DATPRMTIPY EELSGTRHFK GQAQNYSTLL
    61  LEEASARLLV GARGALFSLS ANDIGDGAHK EIHWEASPEM QSKCHQKGKN NQTECFNHVR
   121  FLQRLNSTHL YACGTHAFQP LCAAIDAEAF TLPTSFEEGK EKCPYDPARG FTGLIIDGGL
   181  YTATRYEFRS IPDIRRSRHP HSLRTEETPM HWLNDAEFVF SVLVRESKAS AVGDDDKVYY
   241  FFTERATEEG SGSFTQSRSS HRVARVARVC KGDLGGKKIL QKKWTSFLKA RLICHIPLYE
   301  TLRGVCSLDA ETSSRTHFYA AFTLSTQWKT LEASAICRYD LAEIQAVFAG PYMEYQDGSR
   361  RWGRYEGGVP EPRPGSCITD SLRSQGYNSS QDLPSLVLDF VKLHPLMARP VVPTRGRPLL
   421  LKRNIRYTHL TGTPVTTPAG PTYDLLFLGT ADGWIHKAVV LGSGMHIIEE TQVFRESQSV
   481  ENLVISLLQH SLYVGAPSGV IQLPLSSCSR YRSCYDCILA RDPYCGWDPG THACAAATTI
   541  ANRTALIQDI ERGNRGCESS RDTGPPPPLK TRSVLRGDDV LLPCDQPSNL ARALWLLNGS
   601  MGLSDGQGGY RVGVDGLLVT DAQPEHSGNY GCYAEENGLR TLLASYSLTV RPATPAPAPK
   661  APATPGAQLA PDVRLLYVLA IAALGGLCLI LASSLLYVAC LREGRRGRRR KYSLGRASRA
   721  GGSAVQLQTV SGQCPGEEDE GDDEGAGGLE GSCLQIIPGE GAPAPPPPPP PPPPAELTNG
   781  LVALPSRLRR MNGNSYVLLR QSNNGVPAGP CSFAEELSRI LEKRKHTQLV EQLDESSV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against SEMA4G can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.34
Highest tissue expression
26 nTPM

Expression across tissuesHPA

Tissue

  • rectum: 26 nTPM
  • duodenum: 24 nTPM
  • colon: 20 nTPM
  • small intestine: 19 nTPM
  • liver: 17 nTPM
  • cerebellum: 11 nTPM

Single-cell type

  • colonocytes: 74 nCPM
  • hepatocytes: 72 nCPM
  • enterocytes: 51 nCPM
  • goblet cells: 37 nCPM
  • enteric transient amplifying cells: 36 nCPM
  • paneth cells: 35 nCPM

Immune cell

  • memory B-cell: 0.4 nTPM
  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM

Brain region

  • cerebellum: 17 nTPM
  • thalamus: 16 nTPM
  • midbrain: 13 nTPM
  • choroid plexus: 11 nTPM
  • amygdala: 11 nTPM
  • hypothalamus: 9.3 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about SEMA4G.

Disease | ImmuneIEDB

Conditions an epitope on SEMA4G was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.76
gnomAD pLI
0
gnomAD missense Z
0.95
DepMap mean gene effect
-0.02
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of SEMA4G in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads SEMA4G as an antibody target. Whether an autoantibody or antibody against SEMA4G could matter depends on whether native SEMA4G is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

SEMA4G is annotated at the cell surface, where native SEMA4G is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label SEMA4G as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/SEMA4G. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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