Seroatlas · Human Serome Atlas

RFPL3

Ret finger protein-like 3

Also known as: RFPL3_HUMAN, RNF120

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O75679
Gene: RFPL3
Ensembl: ENSG00000128276
Chromosome: 22
Canonical length: 317 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nuclear speckles,Plasma membrane

OverviewNCBI Gene

Predicted to enable ubiquitin protein ligase activity. Predicted to be involved in innate immune response and regulation of gene expression. Predicted to be located in nucleus. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

317 residues, UniProt reviewed canonical sequence.

>O75679|RFPL3
     1  MKRLSLVTTN RLSPQGNFLP LCTFPLAVDM AALFQEASSC PVCSDYLEKP MSLECGCTVC
    61  LKCINSLQKE PHGEDLLCCC CSMVSQRNKI RPNRQLERLV SHIKELEPKL KKILQMNPRM
   121  RKFQVDMTLD ADTANNFLLI SDDLRSVRSG LITQNRQDLA ERFDVSVCIL GSPRFTCGRH
   181  YWEVDVGTST EWDLGVCRES VHCKGKIQLT TELGFWTVSL RDGSRLSAST VPLTFLLVDR
   241  KLQRVGIFLD MGMQNVSFFD AESGSHVYTF RSVSAEEPLR PFLAPSIPPN GDQGVLSICP
   301  LMNSGTTDAP VRPGEAK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RFPL3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 23 nTPM

Expression across tissuesHPA

Tissue

testis: 23 nTPM
retina: 2.2 nTPM
bone marrow: 1.3 nTPM
skin: 1.2 nTPM
pancreas: 0.7 nTPM
spleen: 0.6 nTPM

Single-cell type

undifferentiated spermatogonia: 9.1 nCPM
late primary spermatocytes: 8.1 nCPM
epicardial cells: 4.5 nCPM
early spermatids: 3.3 nCPM
differentiating spermatogonia: 1.2 nCPM
adipocytes: 0.9 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

cerebellum: 2 nTPM
cerebral cortex: 1.5 nTPM
white matter: 1.4 nTPM
pons: 1.3 nTPM
thalamus: 1.3 nTPM
basal ganglia: 1.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.94
gnomAD pLI: 0
gnomAD missense Z: -0.43
DepMap mean gene effect: 0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RFPL3 as an antibody target. Whether an autoantibody or antibody against RFPL3 could matter depends on whether native RFPL3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RFPL3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RFPL3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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