Seroatlas · Human Serome Atlas

RAD54B

DNA repair and recombination protein RAD54B

Also known as: RA54B_HUMAN, RDH54

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y620
Gene
RAD54B
Ensembl
ENSG00000197275
Chromosome
8
Canonical length
910 aa
Protein class
Cancer-related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

910 residues, UniProt reviewed canonical sequence.

>Q9Y620|RAD54B
     1  MRRSAAPSQL QGNSFKKPKF IPPGRSNPGL NEEITKLNPD IKLFEGVAIN NTFLPSQNDL
    61  RICSLNLPSE ESTREINNRD NCSGKYCFEA PTLATLDPPH TVHSAPKEVA VSKEQEEKSD
   121  SLVKYFSVVW CKPSKKKHKK WEGDAVLIVK GKSFILKNLE GKDIGRGIGY KFKELEKIEE
   181  GQTLMICGKE IEVMGVISPD DFSSGRCFQL GGGSTAISHS SQVARKCFSN PFKSVCKPSS
   241  KENRQNDFQN CKPRHDPYTP NSLVMPRPDK NHQWVFNKNC FPLVDVVIDP YLVYHLRPHQ
   301  KEGIIFLYEC VMGMRMNGRC GAILADEMGL GKTLQCISLI WTLQCQGPYG GKPVIKKTLI
   361  VTPGSLVNNW KKEFQKWLGS ERIKIFTVDQ DHKVEEFIKS IFYSVLIISY EMLLRSLDQI
   421  KNIKFDLLIC DEGHRLKNSA IKTTTALISL SCEKRIILTG TPIQNDLQEF FALIDFVNPG
   481  ILGSLSSYRK IYEEPIILSR EPSASEEEKE LGERRAAELT CLTGLFILRR TQEIINKYLP
   541  PKIENVVFCR PGALQIELYR KLLNSQVVRF CLQGLLENSP HLICIGALKK LCNHPCLLFN
   601  SIKEKECSST CDKNEEKSLY KGLLSVFPAD YNPLLFTEKE SGKLQVLSKL LAVIHELRPT
   661  EKVVLVSNYT QTLNILQEVC KRHGYAYTRL DGQTPISQRQ QIVDGFNSQH SSFFIFLLSS
   721  KAGGVGLNLI GGSHLILYDI DWNPATDIQA MSRVWRDGQK YPVHIYRLLT TGTIEEKIYQ
   781  RQISKQGLCG AVVDLTKTSE HIQFSVEELK NLFTLHESSD CVTHDLLDCE CTGEEVHTGD
   841  SLEKFIVSRD CQLGPHHQKS NSLKPLSMSQ LKQWKHFSGD HLNLTDPFLE RITENVSFIF
   901  QNITTQATGT

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against RAD54B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.35
Highest tissue expression
6.4 nTPM

Expression across tissuesHPA

Tissue

  • retina: 6.4 nTPM
  • fallopian tube: 4.7 nTPM
  • thymus: 3.9 nTPM
  • testis: 3.3 nTPM
  • tonsil: 2.8 nTPM
  • lymph node: 2.5 nTPM

Single-cell type

  • fallopian tube ciliated cells: 35 nCPM
  • erythrocyte progenitors: 29 nCPM
  • megakaryocyte progenitors: 24 nCPM
  • early primary spermatocytes: 22 nCPM
  • differentiating spermatogonia: 18 nCPM
  • retinal pigment epithelial cells: 17 nCPM

Immune cell

  • MAIT T-cell: 1.9 nTPM
  • naive CD8 T-cell: 1.1 nTPM
  • naive B-cell: 1 nTPM
  • memory CD4 T-cell: 0.9 nTPM
  • naive CD4 T-cell: 0.8 nTPM
  • memory B-cell: 0.7 nTPM

Brain region

  • cerebellum: 4.6 nTPM
  • cerebral cortex: 4.5 nTPM
  • basal ganglia: 4.3 nTPM
  • midbrain: 4.2 nTPM
  • choroid plexus: 3.9 nTPM
  • thalamus: 3.8 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about RAD54B.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 146 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Disease | ImmuneIEDB

Conditions an epitope on RAD54B was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.86
gnomAD pLI
0
gnomAD missense Z
0.93
DepMap mean gene effect
-0.08
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of RAD54B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads RAD54B as an antibody target. Whether an autoantibody or antibody against RAD54B could matter depends on whether native RAD54B is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

RAD54B is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label RAD54B as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/RAD54B. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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