POLD3

Protein identityUniProt · HPA

UniProt accession

Q15054

Gene

POLD3

Ensembl

ENSG00000077514

Chromosome

11

Canonical length

466 aa

Protein class

Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]

Canonical amino-acid sequenceUniProt

466 residues, UniProt reviewed canonical sequence.

>Q15054|POLD3
     1  MADQLYLENI DEFVTDQNKI VTYKWLSYTL GVHVNQAKQM LYDYVERKRK ENSGAQLHVT
    61  YLVSGSLIQN GHSCHKVAVV REDKLEAVKS KLAVTASIHV YSIQKAMLKD SGPLFNTDYD
   121  ILKSNLQNCS KFSAIQCAAA VPRAPAESSS SSKKFEQSHL HMSSETQANN ELTTNGHGPP
   181  ASKQVSQQPK GIMGMFASKA AAKTQETNKE TKTEAKEVTN ASAAGNKAPG KGNMMSNFFG
   241  KAAMNKFKVN LDSEQAVKEE KIVEQPTVSV TEPKLATPAG LKKSSKKAEP VKVLQKEKKR
   301  GKRVALSDDE TKETENMRKK RRRIKLPESD SSEDEVFPDS PGAYEAESPS PPPPPSPPLE
   361  PVPKTEPEPP SVKSSSGENK RKRKRVLKSK TYLDGEGCIV TEKVYESESC TDSEEELNMK
   421  TSSVHRPPAM TVKKEPREER KGPKKGTAAL GKANRQVSIT GFFQRK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POLD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.58

Highest tissue expression

40 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about POLD3.

Disease | AllUniProt

Conditions POLD3 is implicated in, by any mechanism.

• Immunodeficiency 122 (IMD122) MIM:620869

Disease | GeneticClinVar

1 pathogenic / likely-pathogenic of 67 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Immunodeficiency 122
• Acute myeloid leukemia
• Nonpapillary renal cell carcinoma
• Ovarian serous cystadenocarcinoma

ReferencesPubMed · IEDB

Publications for POLD3 from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.24

gnomAD pLI

1

gnomAD missense Z

0.09

DepMap mean gene effect

-1.58

DepMap dependency class

pan

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• DNA biosynthetic process
• DNA strand elongation involved in DNA replication
• DNA synthesis involved in DNA repair
• DNA synthesis involved in UV-damage excision repair
• DNA-templated DNA replication
• error-prone translesion synthesis
• mismatch repair
• nucleotide-excision repair, DNA gap filling

Molecular functions

• protein-macromolecule adaptor activity

Cellular components

• cytoplasm
• delta DNA polymerase complex
• nucleoplasm
• zeta DNA polymerase complex

Protein domainsUniProt · Pfam · InterPro

• DNA polymerase delta subunit 3
• DNA polymerase delta subunit 3 superfamily
• DNA polymerase subunit Cdc27

KeywordsUniProt

• Acetylation
• Cytoplasm
• DNA damage
• DNA excision
• DNA repair
• DNA replication
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of POLD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PCNA
• POLD2
• POLD4
• POLDIP2
• REV1
• SPRTN
• POLD1