PBK

Protein identityUniProt · HPA

UniProt accession

Q96KB5

Gene

PBK

Ensembl

ENSG00000168078

Chromosome

8

Canonical length

322 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Cytosol

OverviewNCBI Gene

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

322 residues, UniProt reviewed canonical sequence.

>Q96KB5|PBK
     1  MEGISNFKTP SKLSEKKKSV LCSTPTINIP ASPFMQKLGF GTGVNVYLMK RSPRGLSHSP
    61  WAVKKINPIC NDHYRSVYQK RLMDEAKILK SLHHPNIVGY RAFTEANDGS LCLAMEYGGE
   121  KSLNDLIEER YKASQDPFPA AIILKVALNM ARGLKYLHQE KKLLHGDIKS SNVVIKGDFE
   181  TIKICDVGVS LPLDENMTVT DPEACYIGTE PWKPKEAVEE NGVITDKADI FAFGLTLWEM
   241  MTLSIPHINL SNDDDDEDKT FDESDFDDEA YYAALGTRPP INMEELDESY QKVIELFSVC
   301  TNEDPKDRPS AAHIVEALET DV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PBK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.31

Highest tissue expression

66 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PBK.

Disease | ImmuneIEDB

Conditions an epitope on PBK was assayed in.

• invasive ductal carcinoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.21

gnomAD pLI

0

gnomAD missense Z

0.66

DepMap mean gene effect

0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to UV
• mitotic cell cycle
• negative regulation of inflammatory response
• negative regulation of proteasomal ubiquitin-dependent protein catabolic process
• negative regulation of stress-activated MAPK cascade
• proteasome-mediated ubiquitin-dependent protein catabolic process
• stress-activated MAPK cascade

Molecular functions

• ATP binding
• MAP kinase kinase activity
• protein serine kinase activity
• protein serine/threonine kinase activity
• protein tyrosine kinase activity

Cellular components

• nucleus

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Serine/threonine-protein kinase, active site
• Protein kinase-like domain superfamily
• Protein kinase domain
• TOPK, catalytic domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Isopeptide bond
• Kinase
• Nucleotide-binding
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of PBK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• DLG1
• TP53
• MSL1
• TRIM37