Seroatlas · Human Serome Atlas

NFX1

Transcriptional repressor NF-X1

Also known as: MGC20369, NFX1_HUMAN, NFX2, TEG-42, Tex42

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q12986
Gene: NFX1
Ensembl: ENSG00000086102
Chromosome: 9
Canonical length: 1120 aa
Protein class: Plasma proteins, Predicted intracellular proteins, Transcription factors
Subcellular location: Nucleoplasm,Nucleoli,Plasma membrane,Cytosol

OverviewNCBI Gene

MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1120 residues, UniProt reviewed canonical sequence.

>Q12986|NFX1
     1  MAEAPPVSGT FKFNTDAAEF IPQEKKNSGL NCGTQRRLDS NRIGRRNYSS PPPCHLSRQV
    61  PYDEISAVHQ HSYHPSGSKP KSQQTSFQSS PCNKSPKSHG LQNQPWQKLR NEKHHIRVKK
   121  AQSLAEQTSD TAGLESSTRS ESGTDLREHS PSESEKEVVG ADPRGAKPKK ATQFVYSYGR
   181  GPKVKGKLKC EWSNRTTPKP EDAGPESTKP VGVFHPDSSE ASSRKGVLDG YGARRNEQRR
   241  YPQKRPPWEV EGARPRPGRN PPKQEGHRHT NAGHRNNMGP IPKDDLNERP AKSTCDSENL
   301  AVINKSSRRV DQEKCTVRRQ DPQVVSPFSR GKQNHVLKNV ETHTGSLIEQ LTTEKYECMV
   361  CCELVRVTAP VWSCQSCYHV FHLNCIKKWA RSPASQADGQ SGWRCPACQN VSAHVPNTYT
   421  CFCGKVKNPE WSRNEIPHSC GEVCRKKQPG QDCPHSCNLL CHPGPCPPCP AFMTKTCECG
   481  RTRHTVRCGQ AVSVHCSNPC ENILNCGQHQ CAELCHGGQC QPCQIILNQV CYCGSTSRDV
   541  LCGTDVGKSD GFGDFSCLKI CGKDLKCGNH TCSQVCHPQP CQQCPRLPQL VRCCPCGQTP
   601  LSQLLELGSS SRKTCMDPVP SCGKVCGKPL PCGSLDFIHT CEKLCHEGDC GPCSRTSVIS
   661  CRCSFRTKEL PCTSLKSEDA TFMCDKRCNK KRLCGRHKCN EICCVDKEHK CPLICGRKLR
   721  CGLHRCEEPC HRGNCQTCWQ ASFDELTCHC GASVIYPPVP CGTRPPECTQ TCARVHECDH
   781  PVYHSCHSEE KCPPCTFLTQ KWCMGKHEFR SNIPCHLVDI SCGLPCSATL PCGMHKCQRL
   841  CHKGECLVDE PCKQPCTTPR ADCGHPCMAP CHTSSPCPVT ACKAKVELQC ECGRRKEMVI
   901  CSEASSTYQR IAAISMASKI TDMQLGGSVE ISKLITKKEV HQARLECDEE CSALERKKRL
   961  AEAFHISEDS DPFNIRSSGS KFSDSLKEDA RKDLKFVSDV EKEMETLVEA VNKGKNSKKS
  1021  HSFPPMNRDH RRIIHDLAQV YGLESVSYDS EPKRNVVVTA IRGKSVCPPT TLTGVLEREM
  1081  QARPPPPIPH HRHQSDKNPG SSNLQKITKE PIIDYFDVQD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NFX1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.49
Highest tissue expression: 36 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 36 nTPM
liver: 26 nTPM
tongue: 25 nTPM
thymus: 25 nTPM
skin: 24 nTPM
tonsil: 24 nTPM

Single-cell type

myonuclei: 280 nCPM
pdcs: 201 nCPM
somatotrophs: 182 nCPM
adrenal cortex cells: 181 nCPM
lactotrophs: 167 nCPM
pituicytes/fscs: 160 nCPM

Immune cell

basophil: 36 nTPM
plasmacytoid DC: 16 nTPM
memory B-cell: 13 nTPM
naive CD8 T-cell: 12 nTPM
naive CD4 T-cell: 12 nTPM
memory CD8 T-cell: 11 nTPM

Brain region

cerebellum: 59 nTPM
white matter: 53 nTPM
choroid plexus: 50 nTPM
hypothalamus: 48 nTPM
cerebral cortex: 48 nTPM
basal ganglia: 47 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.46
gnomAD pLI: 0
gnomAD missense Z: 1.41
DepMap mean gene effect: -0.02
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Zinc finger, NF-X1-type
R3H domain
Zinc finger, RING-type
Zinc finger, PHD-finger
Transcription factor NFX1 family
R3H domain superfamily
NF-X1 type zinc finger
R3H domain
Transcriptional repressor NF-X1, R3H domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NFX1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NFX1 as an antibody target. Whether an autoantibody or antibody against NFX1 could matter depends on whether native NFX1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NFX1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NFX1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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