NFIB
Nuclear factor 1 B-type
Also known as: NFI-RED, NFIB_HUMAN, NFIB2, NFIB3
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O00712
- Gene
- NFIB
- Ensembl
- ENSG00000147862
- Chromosome
- 9
- Canonical length
- 420 aa
- Protein class
- Cancer-related genes, Disease related genes, Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm,Nucleoli fibrillar center
- Quaternary structure
- Homodimer
OverviewNCBI Gene
Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and transcription regulator inhibitor activity. Involved in brain development and regulation of DNA-templated transcription. Located in fibrillar center and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
420 residues, UniProt reviewed canonical sequence.
>O00712|NFIB
1 MMYSPICLTQ DEFHPFIEAL LPHVRAIAYT WFNLQARKRK YFKKHEKRMS KDEERAVKDE
61 LLSEKPEIKQ KWASRLLAKL RKDIRQEYRE DFVLTVTGKK HPCCVLSNPD QKGKIRRIDC
121 LRQADKVWRL DLVMVILFKG IPLESTDGER LMKSPHCTNP ALCVQPHHIT VSVKELDLFL
181 AYYVQEQDSG QSGSPSHNDP AKNPPGYLED SFVKSGVFNV SELVRVSRTP ITQGTGVNFP
241 IGEIPSQPYY HDMNSGVNLQ RSLSSPPSSK RPKTISIDEN MEPSPTGDFY PSPSSPAAGS
301 RTWHERDQDM SSPTTMKKPE KPLFSSASPQ DSSPRLSTFP QHHHPGIPGV AHSVISTRTP
361 PPPSPLPFPT QAILPPAPSS YFSHPTIRYP PHLNPQDTLK NYVPSYDPSS PQTSQSWYLGLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NFIB can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.51
- Highest tissue expression
- 38 nTPM
Expression across tissuesHPA
Tissue
- salivary gland: 38 nTPM
- breast: 34 nTPM
- adipose tissue: 27 nTPM
- heart muscle: 24 nTPM
- blood vessel: 23 nTPM
- skin: 21 nTPM
Single-cell type
- salivary myoepithelial cells: 1,651 nCPM
- prostatic glandular cells: 1,231 nCPM
- salivary acinar cells: 1,181 nCPM
- lacrimal acinar cells: 1,111 nCPM
- pituitary stem cells: 1,048 nCPM
- salivary basal cells: 1,034 nCPM
Immune cell
- neutrophil: 0.4 nTPM
- basophil: 0.3 nTPM
- total PBMC: 0.2 nTPM
- MAIT T-cell: 0.1 nTPM
- T-reg: 0.1 nTPM
- classical monocyte: 0 nTPM
Brain region
- cerebellum: 121 nTPM
- cerebral cortex: 101 nTPM
- hippocampal formation: 97 nTPM
- amygdala: 94 nTPM
- spinal cord: 86 nTPM
- basal ganglia: 86 nTPM
DiseaseUniProt · ClinVar · IEDB · PubMed
Four sources answering four different questions about NFIB.
Disease | AllUniProt
Conditions NFIB is implicated in, by any mechanism.
- Macrocephaly, acquired, with impaired intellectual development (MACID) MIM:618286
Disease | GeneticClinVar
35 pathogenic / likely-pathogenic of 199 ClinVar records.
Conditions with pathogenic or likely-pathogenic variants.
- Macrocephaly, acquired, with impaired intellectual development
- Macrocephaly
- Intellectual disability
- Inborn genetic diseases
- Marfanoid habitus and intellectual disability
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.23
- gnomAD pLI
- 1
- gnomAD missense Z
- 2.47
- DepMap mean gene effect
- -0.03
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
- anterior commissure morphogenesis
- brain development
- cell differentiation involved in salivary gland development
- cell proliferation in forebrain
- chondrocyte differentiation
- club cell differentiation
- commissural neuron axon guidance
- DNA replication
- exit from mitosis
- gene expression
- glandular epithelial cell differentiation
- glial cell differentiation
- glial cell fate specification
- glial cell proliferation
- hindbrain development
- negative regulation of DNA binding
- negative regulation of mesenchymal cell proliferation involved in lung development
- negative regulation of miRNA transcription
- negative regulation of stem cell proliferation
- negative regulation of transcription by RNA polymerase II
- neuron fate specification
- positive regulation of DNA-templated transcription
- positive regulation of transcription by RNA polymerase II
- principal sensory nucleus of trigeminal nerve development
- regulation of transcription by RNA polymerase II
- response to bacterium
- response to wounding
- retina development in camera-type eye
- salivary gland cavitation
- stem cell population maintenance
- stem cell proliferation
- tissue homeostasis
- type I pneumocyte differentiation
- type II pneumocyte differentiation
- lung ciliated cell differentiation
- negative regulation of epithelial cell proliferation involved in lung morphogenesis
- regeneration
Molecular functions
- DNA binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
- transcription regulator inhibitor activity
Cellular components
Protein domainsUniProt · Pfam · InterPro
- CTF transcription factor/nuclear factor 1
- MAD homology 1, Dwarfin-type
- CTF transcription factor/nuclear factor 1, N-terminal
- CTF transcription factor/nuclear factor 1, conserved site
- CTF transcription factor/nuclear factor 1, DNA-binding domain
- CTF/NF-I family transcription modulation region
- MH1 domain
- Nuclear factor I protein pre-N-terminus
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NFIB in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NFIB as an antibody target. Whether an autoantibody or antibody against NFIB could matter depends on whether native NFIB is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NFIB is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NFIB as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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