Seroatlas · Human Serome Atlas

PCF11

Pre-mRNA cleavage complex 2 protein Pcf11

Also known as: KIAA0824, PCF11_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O94913
Gene: PCF11
Ensembl: ENSG00000165494
Chromosome: 11
Canonical length: 1555 aa
Protein class: Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Mitochondria

OverviewNCBI Gene

The protein encoded by this gene binds to CLP1 to form pre-mRNA cleavage factor IIm. The encoded protein is necessary for efficient Pol II transcription termination and may be involved in degradation of the 3' product of polyA site cleavage. [provided by RefSeq, Oct 2016]

Canonical amino-acid sequenceUniProt

1555 residues, UniProt reviewed canonical sequence.

>O94913|PCF11
     1  MSEQTPAEAG AAGAREDACR DYQSSLEDLT FNSKPHINML TILAEENLPF AKEIVSLIEA
    61  QTAKAPSSEK LPVMYLMDSI VKNVGREYLT AFTKNLVATF ICVFEKVDEN TRKSLFKLRS
   121  TWDEIFPLKK LYALDVRVNS LDPAWPIKPL PPNVNTSSIH VNPKFLNKSP EEPSTPGTVV
   181  SSPSISTPPI VPDIQKNLTQ EQLIRQQLLA KQKQLLELQQ KKLELELEQA KAQLAVSLSV
   241  QQETSNLGPG SAPSKLHVSQ IPPMAVKAPH QVPVQSEKSR PGPSLQIQDL KGTNRDPRLN
   301  RISQHSHGKD QSHRKEFLMN TLNQSDTKTS KTIPSEKLNS SKQEKSKSGE KITKKELDQL
   361  DSKSKSKSKS PSPLKNKLSH TKDLKNQESE SMRLSDMNKR DPRLKKHLQD KTDGKDDDVK
   421  EKRKTAEKKD KDEHMKSSEH RLAGSRNKII NGIVQKQDTI TEESEKQGTK PGRSSTRKRS
   481  RSRSPKSRSP IIHSPKRRDR RSPKRRQRSM SPTSTPKAGK IRQSGAKQSH MEEFTPPSRE
   541  DRNAKRSTKQ DIRDPRRMKK TEEERPQETT NQHSTKSGTE PKENVENWQS SKSAKRWKSG
   601  WEENKSLQQV DEHSKPPHLR HRESWSSTKG ILSPRAPKQQ QHRLSVDANL QIPKELTLAS
   661  KRELLQKTSE RLASGEITQD DFLVVVHQIR QLFQYQEGVR EEQRSPFNDR FPLKRPRYED
   721  SDKPFVDSPA SRFAGLDTNQ RLTALAEDRP LFDGPSRPSV ARDGPTKMIF EGPNKLSPRI
   781  DGPPTPASLR FDGSPGQMGG GGPLRFEGPQ GQLGGGCPLR FEGPPGPVGT PLRFEGPIGQ
   841  AGGGGFRFEG SPGLRFEGSP GGLRFEGPGG QPVGGLRFEG HRGQPVGGLR FEGPHGQPVG
   901  GLRFDNPRGQ PVGGLRFEGG HGPSGAAIRF DGPHGQPGGG IRFEGPLLQQ GVGMRFEGPH
   961  GQSVAGLRFE GQHNQLGGNL RFEGPHGQPG VGIRFEGPLV QQGGGMRFEG PSVPGGGLRI
  1021  EGPLGQGGPR FEGCHALRFD GQPGQPSLLP RFDGLHGQPG PRFERTPGQP GPQRFDGPPG
  1081  QQVQPRFDGV PQRFDGPQHQ QASRFDIPLG LQGTRFDNHP SQRLESVSFN QTGPYNDPPG
  1141  NAFNAPSQGL QFQRHEQIFD SPQGPNFNGP HGPGNQSFSN PLNRASGHYF DEKNLQSSQF
  1201  GNFGNIPAPM TVGNIQASQQ VLSGVAQPVA FGQGQQFLPV HPQNPGFVQN PSGALPKAYP
  1261  DNHLSQVDVN ELFSKLLKTG ILKLSQTDSA TTQVSEVTAQ PPPEEEEDQN EDQDVPDLTN
  1321  FTVEELKQRY DSVINRLYTG IQCYSCGMRF TTSQTDVYAD HLDWHYRQNR TEKDVSRKVT
  1381  HRRWYYSLTD WIEFEEIADL EERAKSQFFE KVHEEVVLKT QEAAKEKEFQ SVPAGPAGAV
  1441  ESCEICQEQF EQYWDEEEEE WHLKNAIRVD GKIYHPSCYE DYQNTSSFDC TPSPSKTPVE
  1501  NPLNIMLNIV KNELQEPCDS PKVKEERIDT PPACTEESIA TPSEIKTEND TVESV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PCF11 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.6
Highest tissue expression: 31 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 31 nTPM
thymus: 29 nTPM
lymph node: 27 nTPM
tonsil: 26 nTPM
spleen: 25 nTPM
skin: 22 nTPM

Single-cell type

neutrophils: 308 nCPM
early spermatids: 232 nCPM
neutrophil progenitors: 160 nCPM
epididymal basal cells: 147 nCPM
esophageal apical cells: 140 nCPM
b-cells: 129 nCPM

Immune cell

neutrophil: 17 nTPM
eosinophil: 14 nTPM
basophil: 14 nTPM
NK-cell: 13 nTPM
memory B-cell: 10 nTPM
naive B-cell: 9.3 nTPM

Brain region

cerebellum: 68 nTPM
white matter: 44 nTPM
basal ganglia: 39 nTPM
pons: 39 nTPM
medulla oblongata: 38 nTPM
midbrain: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.04
gnomAD pLI: 1
gnomAD missense Z: 2.66
DepMap mean gene effect: -1.04
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

CID domain
ENTH/VHS
CID domain
Pcf11, Clp1-ID
Protein PCF11-like
Pcf11, CID domain
Pre-mRNA cleavage complex 2 protein Pcf11, RFEGP repeat
Pre-mRNA cleavage complex 2 protein Pcf11, helical domain
PCF11, charged region
Pcf11, C-terminal domain
Pcf11, Clp1-interaction domain
PCF11, charged region
Pre-mRNA cleavage complex 2 protein Pcf11, RFEGP repeat
Pre-mRNA cleavage complex 2 protein Pcf11, alpha-helical domain
Pcf11, C-terminal domain

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PCF11 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PCF11 as an antibody target. Whether an autoantibody or antibody against PCF11 could matter depends on whether native PCF11 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PCF11 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PCF11 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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