NECAP2
Adaptin ear-binding coat-associated protein 2
Also known as: FLJ10420, NECP2_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q9NVZ3
- Gene
- NECAP2
- Ensembl
- ENSG00000157191
- Chromosome
- 1
- Canonical length
- 263 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Endoplasmic reticulum
OverviewNCBI Gene
This gene likely encodes a member of the adaptin-ear-binding coat-associated protein family. Studies of a similar protein in rat suggest a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Feb 2009]
Canonical amino-acid sequenceUniProt
263 residues, UniProt reviewed canonical sequence.
>Q9NVZ3|NECAP2
1 MEESGYESVL CVKPDVHVYR IPPRATNRGY RAAEWQLDQP SWSGRLRITA KGQMAYIKLE
61 DRTSGELFAQ APVDQFPGTA VESVTDSSRY FVIRIEDGNG RRAFIGIGFG DRGDAFDFNV
121 ALQDHFKWVK QQCEFAKQAQ NPDQGPKLDL GFKEGQTIKL NIANMKKKEG AAGNPRVRPA
181 STGGLSLLPP PPGGKTSTLI PPPGEQLAVG GSLVQPAVAP SSGGAPVPWP QPNPATADIW
241 GDFTKSTGST SSQTQPGTGW VQFLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NECAP2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Cell surface
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.52
- Highest tissue expression
- 73 nTPM
Expression across tissuesHPA
Tissue
- lymph node: 73 nTPM
- spleen: 72 nTPM
- tonsil: 59 nTPM
- placenta: 56 nTPM
- thymus: 52 nTPM
- appendix: 49 nTPM
Single-cell type
- hofbauer cells: 189 nCPM
- kupffer cells: 167 nCPM
- esophageal apical cells: 154 nCPM
- cone photoreceptor cells: 122 nCPM
- esophageal suprabasal cells: 99 nCPM
- extravillous trophoblasts: 82 nCPM
Immune cell
- non-classical monocyte: 276 nTPM
- total PBMC: 270 nTPM
- intermediate monocyte: 241 nTPM
- T-reg: 215 nTPM
- classical monocyte: 173 nTPM
- naive CD4 T-cell: 169 nTPM
Brain region
- medulla oblongata: 33 nTPM
- thalamus: 30 nTPM
- white matter: 29 nTPM
- spinal cord: 27 nTPM
- basal ganglia: 25 nTPM
- cerebellum: 24 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.45
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.27
- DepMap mean gene effect
- 0
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
InteractionsUniProt · HPA
Protein binding partners of NECAP2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NECAP2 as an antibody target. Whether an autoantibody or antibody against NECAP2 could matter depends on whether native NECAP2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NECAP2 is annotated at the cell surface, where native NECAP2 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.
Annotation status
The present source text does not explicitly label NECAP2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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