NADK

Protein identityUniProt · HPA

UniProt accession

O95544

Gene

NADK

Ensembl

ENSG00000008130

Chromosome

1

Canonical length

446 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Cytosol

OverviewNCBI Gene

NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM, Mar 2008]

Canonical amino-acid sequenceUniProt

446 residues, UniProt reviewed canonical sequence.

>O95544|NADK
     1  MEMEQEKMTM NKELSPDAAA YCCSACHGDE TWSYNHPIRG RAKSRSLSAS PALGSTKEFR
    61  RTRSLHGPCP VTTFGPKACV LQNPQTIMHI QDPASQRLTW NKSPKSVLVI KKMRDASLLQ
   121  PFKELCTHLM EENMIVYVEK KVLEDPAIAS DESFGAVKKK FCTFREDYDD ISNQIDFIIC
   181  LGGDGTLLYA SSLFQGSVPP VMAFHLGSLG FLTPFSFENF QSQVTQVIEG NAAVVLRSRL
   241  KVRVVKELRG KKTAVHNGLG ENGSQAAGLD MDVGKQAMQY QVLNEVVIDR GPSSYLSNVD
   301  VYLDGHLITT VQGDGVIVST PTGSTAYAAA AGASMIHPNV PAIMITPICP HSLSFRPIVV
   361  PAGVELKIML SPEARNTAWV SFDGRKRQEI RHGDSISITT SCYPLPSICV RDPVSDWFES
   421  LAQCLHWNVR KKQAHFEEEE EEEEEG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NADK can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.39

Highest tissue expression

127 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.99

gnomAD pLI

0

gnomAD missense Z

1.88

DepMap mean gene effect

-0.11

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• ATP metabolic process
• NAD+ metabolic process
• NADP+ biosynthetic process
• phosphorylation
• positive regulation of insulin secretion involved in cellular response to glucose stimulus

Molecular functions

• ATP binding
• metal ion binding
• NAD+ kinase activity

Cellular components

• cytosol

Protein domainsUniProt · Pfam · InterPro

• NAD kinase
• NAD kinase/diacylglycerol kinase-like domain superfamily
• ATP-NAD kinase, PpnK-type, C-terminal
• Inorganic polyphosphate/ATP-NAD kinase, N-terminal
• ATP-NAD kinase N-terminal domain
• ATP-NAD kinase C-terminal domain

KeywordsUniProt

• ATP-binding
• Kinase
• Metal-binding
• NAD
• NADP
• Nucleotide-binding
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of NADK in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• YWHAB
• YWHAG
• YWHAH
• YWHAZ