Seroatlas · Human Serome Atlas

MUS81

Structure-specific endonuclease subunit MUS81

Also known as: FLJ44872, MUS81_HUMAN, SLX3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96NY9
Gene
MUS81
Ensembl
ENSG00000172732
Chromosome
11
Canonical length
551 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli fibrillar center

OverviewNCBI Gene

This gene encodes a structure-specific endonuclease which belongs to the XPF/MUS81 endonuclease family and plays a critical role in the resolution of recombination intermediates during DNA repair after inter-strand cross-links, replication fork collapse, and DNA double-strand breaks. The encoded protein associates with one of two closely related essential meiotic endonuclease proteins (EME1 or EME2) to form a complex that processes DNA secondary structures. It contains an N-terminal DEAH helicase domain, an excision repair cross complementation group 4 (ERCC4) endonuclease domain, and two tandem C-terminal helix-hairpin-helix domains. Mice with a homozygous knockout of the orthologous gene have significant meiotic defects including the failure to repair a subset of DNA double strand breaks. [provided by RefSeq, Jun 2017]

Canonical amino-acid sequenceUniProt

551 residues, UniProt reviewed canonical sequence.

>Q96NY9|MUS81
     1  MAAPVRLGRK RPLPACPNPL FVRWLTEWRD EATRSRRRTR FVFQKALRSL RRYPLPLRSG
    61  KEAKILQHFG DGLCRMLDER LQRHRTSGGD HAPDSPSGEN SPAPQGRLAE VQDSSMPVPA
   121  QPKAGGSGSY WPARHSGARV ILLVLYREHL NPNGHHFLTK EELLQRCAQK SPRVAPGSAR
   181  PWPALRSLLH RNLVLRTHQP ARYSLTPEGL ELAQKLAESE GLSLLNVGIG PKEPPGEETA
   241  VPGAASAELA SEAGVQQQPL ELRPGEYRVL LCVDIGETRG GGHRPELLRE LQRLHVTHTV
   301  RKLHVGDFVW VAQETNPRDP ANPGELVLDH IVERKRLDDL CSSIIDGRFR EQKFRLKRCG
   361  LERRVYLVEE HGSVHNLSLP ESTLLQAVTN TQVIDGFFVK RTADIKESAA YLALLTRGLQ
   421  RLYQGHTLRS RPWGTPGNPE SGAMTSPNPL CSLLTFSDFN AGAIKNKAQS VREVFARQLM
   481  QVRGVSGEKA AALVDRYSTP ASLLAAYDAC ATPKEQETLL STIKCGRLQR NLGPALSRTL
   541  SQLYCSYGPL T

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MUS81 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.38
Highest tissue expression
36 nTPM

Expression across tissuesHPA

Tissue

  • endometrium: 36 nTPM
  • cervix: 31 nTPM
  • colon: 31 nTPM
  • fallopian tube: 30 nTPM
  • blood vessel: 30 nTPM
  • ovary: 29 nTPM

Single-cell type

  • late spermatids: 263 nCPM
  • early spermatids: 87 nCPM
  • cardiomyocytes: 48 nCPM
  • epicardial cells: 46 nCPM
  • early primary spermatocytes: 37 nCPM
  • enterocytes: 35 nCPM

Immune cell

  • eosinophil: 28 nTPM
  • basophil: 23 nTPM
  • gdT-cell: 21 nTPM
  • MAIT T-cell: 21 nTPM
  • non-classical monocyte: 19 nTPM
  • memory CD8 T-cell: 18 nTPM

Brain region

  • white matter: 30 nTPM
  • thalamus: 27 nTPM
  • medulla oblongata: 27 nTPM
  • cerebral cortex: 26 nTPM
  • cerebellum: 25 nTPM
  • pons: 25 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.99
gnomAD pLI
0
gnomAD missense Z
0.06
DepMap mean gene effect
-0.32
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MUS81 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MUS81 as an antibody target. Whether an autoantibody or antibody against MUS81 could matter depends on whether native MUS81 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MUS81 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MUS81 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MUS81. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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