EME1

Protein identityUniProt · HPA

UniProt accession

Q96AY2

Gene

EME1

Ensembl

ENSG00000154920

Chromosome

17

Canonical length

570 aa

Protein class

Predicted intracellular proteins

Subcellular location

Nucleoplasm

OverviewNCBI Gene

This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]

Canonical amino-acid sequenceUniProt

570 residues, UniProt reviewed canonical sequence.

>Q96AY2|EME1
     1  MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS DCEASCPPAP
    61  ELFSPPVPEI AETVTQTQPV RLLSSESEDE EEFIPLAQRL TCKFLTHKQL SPEDSSSPVK
   121  SVLDHQNNEG ASCDWKKPFP KIPEVPLHDT PERSAADNKD LILDPCCQLP AYLSTCPGQS
   181  SSLAVTKTNS DILPPQKKTK PSQKVQGRGS HGCRQQRQAR QKESTLRRQE RKNAALVTRM
   241  KAQRPEECLK HIIVVLDPVL LQMEGGGQLL GALQTMECRC VIEAQAVPCS VTWRRRAGPS
   301  EDREDWVEEP TVLVLLRAEA FVSMIDNGKQ GSLDSTMKGK ETLQGFVTDI TAKTAGKALS
   361  LVIVDQEKCF SAQNPPRRGK QGANKQTKKQ QQRQPEASIG SMVSRVDAEE ALVDLQLHTE
   421  AQAQIVQSWK ELADFTCAFT KAVAEAPFKK LRDETTFSFC LESDWAGGVK VDLAGRGLAL
   481  VWRRQIQQLN RVSLEMASAV VNAYPSPQLL VQAYQQCFSD KERQNLLADI QVRRGEGVTS
   541  TSRRIGPELS RRIYLQMTTL QPHLSLDSAD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against EME1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.5

Highest tissue expression

6 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.23

gnomAD pLI

0

gnomAD missense Z

0.08

DepMap mean gene effect

-0.05

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• double-strand break repair
• mitotic intra-S DNA damage checkpoint signaling
• replication fork processing
• resolution of meiotic recombination intermediates
• resolution of mitotic recombination intermediates
• response to intra-S DNA damage checkpoint signaling

Molecular functions

• DNA binding
• metal ion binding
• nuclease activity

Cellular components

• endodeoxyribonuclease complex
• heterochromatin
• Holliday junction resolvase complex
• nuclear chromosome
• nuclear replication fork
• nucleolus
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• ERCC4 domain
• Mms4/EME1/EME2
• EME1/EME2, C-terminal domain
• ERCC4 domain
• EME1/MUS81, C-terminal
• EME1, nuclease domain, subdomain 1
• EME1, nuclease domain, subdomain 2
• Crossover junction endonuclease EME1, XPF-like nuclease domain, vertebrates

KeywordsUniProt

• DNA damage
• DNA recombination
• DNA repair
• Isopeptide bond
• Magnesium
• Metal-binding
• Nucleus
• Phosphoprotein
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of EME1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MUS81
• SLX4