MRPS28

Protein identityUniProt · HPA

UniProt accession

Q9Y2Q9

Gene

MRPS28

Ensembl

ENSG00000147586

Chromosome

8

Canonical length

187 aa

Protein class

Disease related genes, Human disease related genes, Predicted intracellular proteins, Ribosomal proteins

Subcellular location

Mitochondria

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

187 residues, UniProt reviewed canonical sequence.

>Q9Y2Q9|MRPS28
     1  MAALCRTRAV AAESHFLRVF LFFRPFRGVG TESGSESGSS NAKEPKTRAG GFASALERHS
    61  ELLQKVEPLQ KGSPKNVESF ASMLRHSPLT QMGPAKDKLV IGRIFHIVEN DLYIDFGGKF
   121  HCVCRRPEVD GEKYQKGTRV RLRLLDLELT SRFLGATTDT TVLEANAVLL GIQESKDSRS
   181  KEEHHEK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPS28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.52

Highest tissue expression

150 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MRPS28.

Disease | AllUniProt

Conditions MRPS28 is implicated in, by any mechanism.

• Combined oxidative phosphorylation deficiency 47 (COXPD47) MIM:618958

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 56 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Combined oxidative phosphorylation deficiency 47

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.88

gnomAD pLI

0

gnomAD missense Z

-0.03

DepMap mean gene effect

-0.49

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 17% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mitochondrial translation

Molecular functions

• RNA binding

Cellular components

• mitochondrial inner membrane
• mitochondrial small ribosomal subunit
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Small ribosomal subunit protein bS1m
• Mitochondrial ribosomal protein MRP-S35

KeywordsUniProt

• Acetylation
• Mitochondrion
• Phosphoprotein
• Primary mitochondrial disease
• Ribonucleoprotein
• Ribosomal protein
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of MRPS28 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MRPS18B
• MRPS2
• MRPS23
• MRPS26
• MRPS27