METAP2

Protein identityUniProt · HPA

UniProt accession

P50579

Gene

METAP2

Ensembl

ENSG00000111142

Chromosome

12

Canonical length

478 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Canonical amino-acid sequenceUniProt

478 residues, UniProt reviewed canonical sequence.

>P50579|METAP2
     1  MAGVEEVAAS GSHLNGDLDP DDREEGAAST AEEAAKKKRR KKKKSKGPSA AGEQEPDKES
    61  GASVDEVARQ LERSALEDKE RDEDDEDGDG DGDGATGKKK KKKKKKRGPK VQTDPPSVPI
   121  CDLYPNGVFP KGQECEYPPT QDGRTAAWRT TSEEKKALDQ ASEEIWNDFR EAAEAHRQVR
   181  KYVMSWIKPG MTMIEICEKL EDCSRKLIKE NGLNAGLAFP TGCSLNNCAA HYTPNAGDTT
   241  VLQYDDICKI DFGTHISGRI IDCAFTVTFN PKYDTLLKAV KDATNTGIKC AGIDVRLCDV
   301  GEAIQEVMES YEVEIDGKTY QVKPIRNLNG HSIGQYRIHA GKTVPIVKGG EATRMEEGEV
   361  YAIETFGSTG KGVVHDDMEC SHYMKNFDVG HVPIRLPRTK HLLNVINENF GTLAFCRRWL
   421  DRLGESKYLM ALKNLCDLGI VDPYPPLCDI KGSYTAQFEH TILLRPTCKE VVSRGDDY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against METAP2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

117 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.59

gnomAD pLI

0

gnomAD missense Z

2.94

DepMap mean gene effect

-0.63

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• protein processing
• regulation of translational initiation

Molecular functions

• aminopeptidase activity
• initiator methionyl aminopeptidase activity
• metal ion binding
• metalloaminopeptidase activity
• metalloexopeptidase activity
• RNA binding

Cellular components

• cytoplasm
• cytosol
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Peptidase M24
• Peptidase M24, methionine aminopeptidase
• Peptidase M24A, methionine aminopeptidase, subfamily 2, binding site
• Creatinase/aminopeptidase-like
• Winged helix-like DNA-binding domain superfamily
• Winged helix DNA-binding domain superfamily
• Metallopeptidase family M24
• Peptidase M24A, methionine aminopeptidase, subfamily 2
• Methionine Aminopeptidase Type 2

KeywordsUniProt

• Acetylation
• Aminopeptidase
• Cytoplasm
• Glycoprotein
• Hydrolase
• Metal-binding
• Phosphoprotein
• Protease

InteractionsUniProt · HPA

Protein binding partners of METAP2 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CCT2
• STAU1