Seroatlas · Human Serome Atlas

MAST3

Microtubule-associated serine/threonine-protein kinase 3

Also known as: KIAA0561, MAST3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O60307
Gene: MAST3
Ensembl: ENSG00000099308
Chromosome: 19
Canonical length: 1309 aa
Protein class: Enzymes, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nuclear speckles

OverviewNCBI Gene

Predicted to enable protein serine/threonine kinase activity. Predicted to be involved in cytoskeleton organization and intracellular signal transduction. Predicted to be located in cytoplasm. Implicated in developmental and epileptic encephalopathy 108. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1309 residues, UniProt reviewed canonical sequence.

>O60307|MAST3
     1  MDESSLLRRR GLQKELSLPR RGRGCRSGNR KSLVVGTPSP TLSRPLSPLS VPTAGSSPLD
    61  SPRNFSAASA LNFPFARRAD GRRWSLASLP SSGYGTNTPS STLSSSSSSR ERLHQLPFQP
   121  TPDELHFLSK HFRSSENVLD EEGGRSPRLR PRSRSLSPGR ATGTFDNEIV MMNHVYRERF
   181  PKATAQMEGR LQEFLTAYAP GARLALADGV LGFIHHQIVE LARDCLAKSG ENLVTSRYFL
   241  EMQEKLERLL QDAHERSDSE EVSFIVQLVR KLLIIISRPA RLLECLEFDP EEFYHLLEAA
   301  EGHAREGQGI KTDLPQYIIG QLGLAKDPLE EMVPLSHLEE EQPPAPESPE SRALVGQSRR
   361  KPCESDFETI KLISNGAYGA VYLVRHRDTR QRFAIKKINK QNLILRNQIQ QVFVERDILT
   421  FAENPFVVSM FCSFETRRHL CMVMEYVEGG DCATLLKNMG PLPVDMARLY FAETVLALEY
   481  LHNYGIVHRD LKPDNLLITS LGHIKLTDFG LSKIGLMSMA TNLYEGHIEK DAREFIDKQV
   541  CGTPEYIAPE VIFRQGYGKP VDWWAMGVVL YEFLVGCVPF FGDTPEELFG QVVSDEIMWP
   601  EGDEALPADA QDLITRLLRQ SPLDRLGTGG THEVKQHPFF LALDWAGLLR HKAEFVPQLE
   661  AEDDTSYFDT RSERYRHLGS EDDETNDEES STEIPQFSSC SHRFSKVYSS SEFLAVQPTP
   721  TFAERSFSED REEGWERSEV DYGRRLSADI RLRSWTSSGS SCQSSSSQPE RGPSPSLLNT
   781  ISLDTMPKFA FSSEDEGVGP GPAGPKRPVF ILGEPDPPPA ATPVMPKPSS LSADTAALSH
   841  ARLRSNSIGA RHSTPRPLDA GRGRRLGGPR DPAPEKSRAS SSGGSGGGSG GRVPKSASVS
   901  ALSLIITADD GSGGPLMSPL SPRSLSSNPS SRDSSPSRDP SPVCGSLRPP IVIHSSGKKY
   961  GFSLRAIRVY MGDSDVYTVH HVVWSVEDGS PAQEAGLRAG DLITHINGES VLGLVHMDVV
  1021  ELLLKSGNKI SLRTTALENT SIKVGPARKN VAKGRMARRS KRSRRRETQD RRKSLFKKIS
  1081  KQTSVLHTSR SFSSGLHHSL SSSESLPGSP THSLSPSPTT PCRSPAPDVP ADTTASPPSA
  1141  SPSSSSPASP AAAGHTRPSS LHGLAAKLGP PRPKTGRRKS TSSIPPSPLA CPPISAPPPR
  1201  SPSPLPGHPP APARSPRLRR GQSADKLGTG ERLDGEAGRR TRGPEAELVV MRRLHLSERR
  1261  DSFKKQEAVQ EVSFDEPQEE ATGLPTSVPQ IAVEGEEAVP VALGPTGRD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAST3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.5
Highest tissue expression: 45 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 45 nTPM
hippocampal formation: 30 nTPM
basal ganglia: 29 nTPM
amygdala: 27 nTPM
pancreas: 15 nTPM
bone marrow: 15 nTPM

Single-cell type

neutrophils: 379 nCPM
neutrophil progenitors: 86 nCPM
oligodendrocytes: 85 nCPM
microglia: 69 nCPM
pdcs: 64 nCPM
brain excitatory neurons: 54 nCPM

Immune cell

neutrophil: 33 nTPM
eosinophil: 17 nTPM
plasmacytoid DC: 17 nTPM
non-classical monocyte: 16 nTPM
gdT-cell: 12 nTPM
MAIT T-cell: 9.2 nTPM

Brain region

cerebral cortex: 231 nTPM
hippocampal formation: 225 nTPM
basal ganglia: 203 nTPM
amygdala: 197 nTPM
white matter: 192 nTPM
thalamus: 100 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAST3.

Disease | AllUniProt

Conditions MAST3 is implicated in, by any mechanism.

Developmental and epileptic encephalopathy 108 (DEE108) MIM:620115

Disease | GeneticClinVar

9 pathogenic / likely-pathogenic of 324 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Developmental and epileptic encephalopathy 108
Inborn genetic diseases
Pervasive developmental disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.22
gnomAD pLI: 1
gnomAD missense Z: 4.14
DepMap mean gene effect: -0.14
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAST3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAST3 as an antibody target. Whether an autoantibody or antibody against MAST3 could matter depends on whether native MAST3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAST3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MAST3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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