KLHL28
Kelch-like protein 28
Also known as: BTBD5, FLJ20081, KLH28_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q9NXS3
- Gene
- KLHL28
- Ensembl
- ENSG00000179454
- Chromosome
- 14
- Canonical length
- 571 aa
- Protein class
- Predicted intracellular proteins
- Subcellular location
- Cytosol
OverviewNCBI Gene
Predicted to enable ubiquitin-like ligase-substrate adaptor activity. Predicted to be involved in proteasome-mediated ubiquitin-dependent protein catabolic process. Predicted to be part of Cul3-RING ubiquitin ligase complex. Predicted to be active in cytoplasm. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
571 residues, UniProt reviewed canonical sequence.
>Q9NXS3|KLHL28
1 MDHTSPTYML ANLTHLHSEQ LLQGLNLLRQ HHELCDIILR VGDVKIHAHK VVLASVSPYF
61 KAMFTGNLSE KENSEVEFQC IDETALQAIV EYAYTGTVFI SQDTVESLLP AANLLQIKLV
121 LKECCAFLES QLDPGNCIGI SRFAETYGCR DLYLAATKYI CQNFEAVCQT EEFFELTHAD
181 LDEIVSNDCL NVATEETVFY ALESWIKYDV QERQKYLAQL LNSVRLPLLS VKFLTRLYEA
241 NHLIRDDRTC KHLLNEALKY HFMPEHRLSH QTVLMTRPRC APKVLCAVGG KSGLFACLDS
301 VEMYFPQNDS WIGLAPLNIP RYEFGICVLD QKVYVIGGIA TNVRPGVTIR KHENSVECWN
361 PDTNTWTSLE RMNESRSTLG VVVLAGELYA LGGYDGQSYL QSVEKYIPKI RKWQPVAPMT
421 TTRSCFAAAV LDGMIYAIGG YGPAHMNSVE RYDPSKDSWE MVASMADKRI HFGVGVMLGF
481 IFVVGGHNGV SHLSSIERYD PHQNQWTVCR PMKEPRTGVG AAVIDNYLYV VGGHSGSSYL
541 NTVQKYDPIS DTWLDSAGMI YCRCNFGLTA LLocalizationUniProt · AlphaFold · HPA
Whether an antibody against KLHL28 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.25
- Highest tissue expression
- 26 nTPM
Expression across tissuesHPA
Tissue
- bone marrow: 26 nTPM
- thymus: 8.5 nTPM
- lymph node: 8.1 nTPM
- retina: 7.5 nTPM
- tongue: 7.4 nTPM
- thyroid gland: 7.2 nTPM
Single-cell type
- cardiomyocytes: 619 nCPM
- cone photoreceptor cells: 267 nCPM
- rod photoreceptor cells: 195 nCPM
- myonuclei: 188 nCPM
- epicardial cells: 171 nCPM
- endometrial ciliated cells: 144 nCPM
Immune cell
- basophil: 32 nTPM
- MAIT T-cell: 32 nTPM
- NK-cell: 20 nTPM
- naive CD4 T-cell: 20 nTPM
- eosinophil: 18 nTPM
- memory CD8 T-cell: 18 nTPM
Brain region
- cerebellum: 32 nTPM
- white matter: 26 nTPM
- hypothalamus: 21 nTPM
- thalamus: 21 nTPM
- pons: 21 nTPM
- cerebral cortex: 21 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 0.59
- gnomAD pLI
- 0.02
- gnomAD missense Z
- 2.2
- DepMap mean gene effect
- 0.02
- DepMap dependency class
- none
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads KLHL28 as an antibody target. Whether an autoantibody or antibody against KLHL28 could matter depends on whether native KLHL28 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
KLHL28 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label KLHL28 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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