Seroatlas · Human Serome Atlas

KLHL25

Kelch-like protein 25

Also known as: ENC-2, ENC2, FLJ12587, KLH25_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9H0H3
Gene
KLHL25
Ensembl
ENSG00000183655
Chromosome
15
Canonical length
589 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; regulation of fatty acid metabolic process; and ubiquitin-dependent protein catabolic process. Located in cytoplasm. Part of Cul3-RING ubiquitin ligase complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

589 residues, UniProt reviewed canonical sequence.

>Q9H0H3|KLHL25
     1  MSVSVHETRK SRSSTGSMNV TLFHKASHPD CVLAHLNTLR KHCMFTDVTL WAGDRAFPCH
    61  RAVLAASSRY FEAMFSHGLR ESRDDTVNFQ DNLHPEVLEL LLDFAYSSRI AINEENAESL
   121  LEAGDMLQFH DVRDAAAEFL EKNLFPSNCL GMMLLSDAHQ CRRLYEFSWR MCLVHFETVR
   181  QSEDFNSLSK DTLLDLISSD ELETEDERVV FEAILQWVKH DLEPRKVHLP ELLRSVRLAL
   241  LPSDCLQEAV SSEALLMADE RTKLIMDEAL RCKTRILQND GVVTSPCARP RKAGHTLLIL
   301  GGQTFMCDKI YQVDHKAKEI IPKADLPSPR KEFSASAIGC KVYVTGGRGS ENGVSKDVWV
   361  YDTVHEEWSK AAPMLIARFG HGSAELENCL YVVGGHTSLA GVFPASPSVS LKQVEKYDPG
   421  ANKWMMVAPL RDGVSNAAVV SAKLKLFVFG GTSIHRDMVS KVQCYDPSEN RWTIKAECPQ
   481  PWRYTAAAVL GSQIFIMGGD TEFTAASAYR FDCETNQWTR IGDMTAKRMS CHALASGNKL
   541  YVVGGYFGTQ RCKTLDCYDP TSDTWNCITT VPYSLIPTAF VSTWKHLPA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against KLHL25 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.26
Highest tissue expression
15 nTPM

Expression across tissuesHPA

Tissue

  • liver: 15 nTPM
  • testis: 10 nTPM
  • skin: 9.2 nTPM
  • cerebral cortex: 8.7 nTPM
  • basal ganglia: 7.3 nTPM
  • skeletal muscle: 6.4 nTPM

Single-cell type

  • breast lactating cells: 32 nCPM
  • pancreatic islet cells: 31 nCPM
  • salivary myoepithelial cells: 29 nCPM
  • gonadotrophs: 27 nCPM
  • adipocytes: 27 nCPM
  • hepatocytes: 25 nCPM

Immune cell

  • non-classical monocyte: 3.9 nTPM
  • NK-cell: 2.6 nTPM
  • naive CD4 T-cell: 1.7 nTPM
  • intermediate monocyte: 1.2 nTPM
  • naive CD8 T-cell: 1.1 nTPM
  • gdT-cell: 1 nTPM

Brain region

  • midbrain: 16 nTPM
  • hypothalamus: 14 nTPM
  • basal ganglia: 14 nTPM
  • thalamus: 14 nTPM
  • cerebral cortex: 14 nTPM
  • pons: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.92
gnomAD pLI
0
gnomAD missense Z
-1.43
DepMap mean gene effect
0.04
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of KLHL25 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads KLHL25 as an antibody target. Whether an autoantibody or antibody against KLHL25 could matter depends on whether native KLHL25 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

KLHL25 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label KLHL25 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/KLHL25. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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