Seroatlas · Human Serome Atlas

HIF3A

Hypoxia-inducible factor 3-alpha

Also known as: bHLHe17, HIF3A_HUMAN, IPAS, MOP7, PASD7

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9Y2N7
Gene
HIF3A
Ensembl
ENSG00000124440
Chromosome
19
Canonical length
669 aa
Protein class
Predicted intracellular proteins, Transcription factors
Subcellular location
Nucleoplasm,Plasma membrane,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is the alpha-3 subunit of one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha-3 subunit lacks the transactivation domain found in factors containing either the alpha-1 or alpha-2 subunits. It is thought that factors containing the alpha-3 subunit are negative regulators of hypoxia-inducible gene expression. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2011]

Canonical amino-acid sequenceUniProt

669 residues, UniProt reviewed canonical sequence.

>Q9Y2N7|HIF3A
     1  MALGLQRARS TTELRKEKSR DAARSRRSQE TEVLYQLAHT LPFARGVSAH LDKASIMRLT
    61  ISYLRMHRLC AAGEWNQVGA GGEPLDACYL KALEGFVMVL TAEGDMAYLS ENVSKHLGLS
   121  QLELIGHSIF DFIHPCDQEE LQDALTPQQT LSRRKVEAPT ERCFSLRMKS TLTSRGRTLN
   181  LKAATWKVLN CSGHMRAYKP PAQTSPAGSP DSEPPLQCLV LICEAIPHPG SLEPPLGRGA
   241  FLSRHSLDMK FTYCDDRIAE VAGYSPDDLI GCSAYEYIHA LDSDAVSKSI HTLLSKGQAV
   301  TGQYRFLARS GGYLWTQTQA TVVSGGRGPQ SESIVCVHFL ISQVEETGVV LSLEQTEQHS
   361  RRPIQRGAPS QKDTPNPGDS LDTPGPRILA FLHPPSLSEA ALAADPRRFC SPDLRRLLGP
   421  ILDGASVAAT PSTPLATRHP QSPLSADLPD ELPVGTENVH RLFTSGKDTE AVETDLDIAQ
   481  DADALDLEML APYISMDDDF QLNASEQLPR AYHRPLGAVP RPRARSFHGL SPPALEPSLL
   541  PRWGSDPRLS CSSPSRGDPS ASSPMAGARK RTLAQSSEDE DEGVELLGVR PPKRSPSPEH
   601  ENFLLFPLSL SFLLTGGPAP GSLQDPSTPL LNLNEPLGLG PSLLSPYSDE DTTQPGGPFQ
   661  PRAGSAQAD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HIF3A can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.48
Highest tissue expression
56 nTPM

Expression across tissuesHPA

Tissue

  • urinary bladder: 56 nTPM
  • heart muscle: 53 nTPM
  • colon: 53 nTPM
  • blood vessel: 38 nTPM
  • stomach: 35 nTPM
  • endometrium: 34 nTPM

Single-cell type

  • astrocytes: 513 nCPM
  • bergmann glia: 492 nCPM
  • leydig cells: 466 nCPM
  • peritubular myoid cells: 346 nCPM
  • müller glia: 335 nCPM
  • hematopoietic stem cells: 315 nCPM

Immune cell

  • basophil: 0.9 nTPM
  • neutrophil: 0.7 nTPM
  • plasmacytoid DC: 0.7 nTPM
  • non-classical monocyte: 0.4 nTPM
  • eosinophil: 0.2 nTPM
  • naive B-cell: 0.2 nTPM

Brain region

  • basal ganglia: 41 nTPM
  • amygdala: 40 nTPM
  • cerebral cortex: 38 nTPM
  • midbrain: 37 nTPM
  • thalamus: 36 nTPM
  • medulla oblongata: 36 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.6
gnomAD pLI
0
gnomAD missense Z
0.76
DepMap mean gene effect
0.16
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of HIF3A in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads HIF3A as an antibody target. Whether an autoantibody or antibody against HIF3A could matter depends on whether native HIF3A is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

HIF3A is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label HIF3A as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/HIF3A. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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