HECTD3

Protein identityUniProt · HPA

UniProt accession

Q5T447

Gene

HECTD3

Ensembl

ENSG00000126107

Chromosome

1

Canonical length

861 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

The protein encoded by this gene transfers ubiquitin from an E2 ubiquitin-conjugating enzyme to targeted substrates, leading to the degradation of those substrates. The encoded protein has been shown to transfer ubiquitin to TRIOBP to facilitate cell cycle progression, and to STX8. [provided by RefSeq, Dec 2012]

Canonical amino-acid sequenceUniProt

861 residues, UniProt reviewed canonical sequence.

>Q5T447|HECTD3
     1  MAGPGPGAVL ESPRQLLGRV RFLAEAARSL RAGRPLPAAL AFVPREVLYK LYKDPAGPSR
    61  VLLPVWEAEG LGLRVGAAGP APGTGSGPLR AARDSIELRR GACVRTTGEE LCNGHGLWVK
   121  LTKEQLAEHL GDCGLQEGWL LVCRPAEGGA RLVPIDTPNH LQRQQQLFGV DYRPVLRWEQ
   181  VVDLTYSHRL GSRPQPAEAY AEAVQRLLYV PPTWTYECDE DLIHFLYDHL GKEDENLGSV
   241  KQYVESIDVS SYTEEFNVSC LTDSNADTYW ESDGSQCQHW VRLTMKKGTI VKKLLLTVDT
   301  TDDNFMPKRV VVYGGEGDNL KKLSDVSIDE TLIGDVCVLE DMTVHLPIIE IRIVECRDDG
   361  IDVRLRGVKI KSSRQRELGL NADLFQPTSL VRYPRLEGTD PEVLYRRAVL LQRFIKILDS
   421  VLHHLVPAWD HTLGTFSEIK QVKQFLLLSR QRPGLVAQCL RDSESSKPSF MPRLYINRRL
   481  AMEHRACPSR DPACKNAVFT QVYEGLKPSD KYEKPLDYRW PMRYDQWWEC KFIAEGIIDQ
   541  GGGFRDSLAD MSEELCPSSA DTPVPLPFFV RTANQGNGTG EARDMYVPNP SCRDFAKYEW
   601  IGQLMGAALR GKEFLVLALP GFVWKQLSGE EVSWSKDFPA VDSVLVKLLE VMEGMDKETF
   661  EFKFGKELTF TTVLSDQQVV ELIPGGAGIV VGYGDRSRFI QLVQKARLEE SKEQVAAMQA
   721  GLLKVVPQAV LDLLTWQELE KKVCGDPEVT VDALRKLTRF EDFEPSDSRV QYFWEALNNF
   781  TNEDRSRFLR FVTGRSRLPA RIYIYPDKLG YETTDALPES STCSSTLFLP HYASAKVCEE
   841  KLRYAAYNCV AIDTDMSPWE E

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against HECTD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

44 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.4

gnomAD pLI

0.09

gnomAD missense Z

2.15

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• proteasome-mediated ubiquitin-dependent protein catabolic process
• protein ubiquitination

Molecular functions

• syntaxin binding
• ubiquitin protein ligase activity
• ubiquitin-protein transferase activity

Cellular components

• perinuclear region of cytoplasm

Protein domainsUniProt · Pfam · InterPro

• HECT domain
• APC10/DOC domain
• Galactose-binding-like domain superfamily
• HECT, E3 ligase catalytic domain
• HECT-domain (ubiquitin-transferase)
• Anaphase-promoting complex, subunit 10 (APC10)
• E3 ubiquitin-protein ligase HECTD3

KeywordsUniProt

• Acetylation
• Cytoplasm
• Phosphoprotein
• Transferase
• Ubl conjugation pathway

InteractionsUniProt · HPA

Protein binding partners of HECTD3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• STX8
• TRIOBP
• SLC7A11