Seroatlas · Human Serome Atlas

H3-4

Histone H3.1t

Also known as: H3.4, H3/g, H31T_HUMAN, H3C16, H3FT, H3t, HIST3H3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q16695
Gene
H3-4
Ensembl
ENSG00000168148
Chromosome
1
Canonical length
136 aa
Protein class
Predicted intracellular proteins
Subcellular location
Nucleoplasm,Acrosome,Principal piece

OverviewNCBI Gene

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]

Canonical amino-acid sequenceUniProt

136 residues, UniProt reviewed canonical sequence.

>Q16695|H3-4
     1  MARTKQTARK STGGKAPRKQ LATKVARKSA PATGGVKKPH RYRPGTVALR EIRRYQKSTE
    61  LLIRKLPFQR LMREIAQDFK TDLRFQSSAV MALQEACESY LVGLFEDTNL CVIHAKRVTI
   121  MPKDIQLARR IRGERA

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against H3-4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.53
Highest tissue expression
3.3 nTPM

Expression across tissuesHPA

Tissue

  • testis: 3.3 nTPM
  • cerebellum: 0.2 nTPM
  • blood vessel: 0.1 nTPM
  • cervix: 0.1 nTPM
  • colon: 0.1 nTPM
  • endometrium: 0.1 nTPM

Single-cell type

  • adipocytes: 0 nCPM
  • adrenal cortex cells: 0 nCPM
  • adrenal medulla cells: 0 nCPM
  • alveolar cells type 1: 0 nCPM
  • alveolar cells type 2: 0 nCPM
  • astrocytes: 0 nCPM

Immune cell

  • basophil: 0 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • cerebellum: 4.6 nTPM
  • choroid plexus: 3.3 nTPM
  • pons: 3.1 nTPM
  • cerebral cortex: 2.7 nTPM
  • medulla oblongata: 2.5 nTPM
  • thalamus: 2 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about H3-4.

Disease | ImmuneIEDB

Conditions an epitope on H3-4 was assayed in.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.94
gnomAD pLI
0
DepMap mean gene effect
-0.22
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of H3-4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads H3-4 as an antibody target. Whether an autoantibody or antibody against H3-4 could matter depends on whether native H3-4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

H3-4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label H3-4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/H3-4. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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